Fluorometer FAQs
Fluorometer - Frequently Asked Questions
What are the differences between the Fluorometer models?
The Fluorometer Series includes four models designed for different throughput requirements. Fluorometer 1 supports single-sample testing for DNA, RNA and protein quantification. Fluorometer 8 (DNA) supports up to 8 samples for DNA and protein detection, while Fluorometer 8 (DNA&RNA) adds RNA detection with dual-path fluorescence. Fluorometer 96 is designed for high-throughput workflows with support for up to 96 samples per run.
Which consumables should be used with the Fluorometer Series?
Each Fluorometer model requires specific consumables for optimal performance. Fluorometer 1 uses 0.5 mL transparent flat-cap PCR tubes, Fluorometer 8 models use 0.2 mL transparent PCR 8-strip tubes, and Fluorometer 96 uses 0.3 mL black flat-bottomed microplates with a height below 15 mm.
Why is the fluorometer not responding after powering on?
If the instrument does not power on, check that the power cable is securely connected and confirm that the instrument is connected to a stable power source. It is also recommended to use the original power cord supplied with the instrument.
Why are my concentration measurement results inaccurate?
Inaccurate quantification results may be caused by incorrect sample preparation, pipetting errors, unsuitable standards or inconsistent reagent handling. For best results, ensure reagents are fully mixed, samples are prepared accurately and the instrument is operated in a stable environment free from strong light or temperature fluctuations.
Do I need to prepare a new calibration curve for every experiment?
Preparing a new calibration curve is recommended whenever new reagents are prepared or when high accuracy is required. Existing calibration curves may still be used if experimental conditions remain stable and previous results are consistent.
Which excitation light should be used for different sample types?
Blue excitation light is used for dsDNA, ssDNA, microRNA and protein assays, while red excitation light is used for RNA HS, RNA BR and RNA ER assays.
How long can prepared standards and reagents be stored?
Prepared standards should ideally be used within 1 hour unless otherwise specified in the reagent instructions. Reagents should not remain at room temperature for more than 3 hours to maintain reliable fluorescence performance.
Why does the fluorescence signal decrease during repeated measurements?
Fluorescence signals may decrease due to photobleaching, which occurs when fluorescent dyes are repeatedly exposed to excitation light. To reduce this effect, avoid repeated consecutive measurements of the same sample whenever possible.
What is the fluorescence detection range of the Fluorometer Series?
The fluorescence upper detection threshold depends on the model. Fluorometer 1 supports measurements up to millions of RFU, Fluorometer 8 (DNA) supports up to 300,000 RFU, while Fluorometer 8 (DNA&RNA) and Fluorometer 96 support up to 60,000 RFU.
What excitation and emission wavelengths are supported by the Fluorometer Series?
The Fluorometer Series supports excitation wavelength ranges around 460–480 nm and 630–650 nm, with emission wavelength ranges around 500–535 nm and 670–710 nm depending on the model, enabling sensitive DNA, RNA and protein detection.
