G400 FAQs
G400 - Frequently Asked Questions
What is the difference between DNBSEQ-G400 and MGISEQ-2000?
They are the same instrument. The product model names DNBSEQ-G400 and MGISEQ-2000 are used in selected overseas countries and China, respectively.
What are StandardMPS 2.0 (SM2.0) sequencing reagents?
The StandardMPS 2.0 Reagent Set (SM2.0) is an upgraded version of the existing StandardMPS Reagent Set (SM). It uses DNA polymerases with higher polymerization efficiency to improve signal intensity and stability. The Q-value table is trained with data closer to actual estimates, resulting in more accurate data interpretation and Q40 > 85%.
Can SM and SM2.0 reagents be used together in the same run?
No. SM and SM2.0 reagents cannot be used in the same sequencing run.
What is the difference between the initial SM2.0 (SM+) version and the global SM2.0 version?
The initial version released in the UK, Germany, Switzerland, Sweden, and Hong Kong is internally referred to as SM+. The global version is referred to as SM2.0. SM2.0 is further optimized and includes App-D integrated sequencing reagents compatible with third-party libraries, in addition to MGI libraries.
How do I know which SM2.0 reagent version applies to my region?
The officially released SM2.0 reagent version follows the SM reagent naming convention. In user manuals and product labels, the English name adds “Reagent” after “Sequencing”. Versions differ by region (e.g., V4.0 / V5.0 for certain markets, V1.0 for App-D reagents). Refer to the regional product name and model tables for exact details.
What software and reagent compatibility should I be aware of?
Compatibility has evolved across ECR versions (ECR1.0 to ECR7.1+). Newer control software (ZebraV2Seq) and algorithm software (BasecallLite) support both SM and SM2.0 reagents. Always check the latest release notes for your instrument’s upgrade code to ensure full compatibility.
Will SM2.0 reagents produce smaller data files?
Yes. Due to improved data quality and more concentrated Q-value distribution with SM2.0 reagents, fq data compresses more efficiently, resulting in smaller file sizes for the same amount of data.
Can I still order StandardMPS (SM) reagents?
Yes. In the future, FCS SM reagents, FCL SM reagents, and FCL SM2.0 reagents will continue to be supplied. FCS SM2.0 reagents will not be released.
Which user manual should I use with SM2.0 reagents?
Refer to the latest versions:
CE-IVD: H-020-000812-00 2.0 Universal Sequencing Reaction Kit Instructions for Use (G400 FCL CE)
CE-RUO: Sequencer + Software + Reagent Three-in-One User Manual (H-020-000780-00 2.0 DNBSEQ-G400RS System Guide)
What are the key IVD qualification updates for SM2.0 reagents?
Both NMPA and CE-IVD use the strategy of adding models without changing the certificate number. All FCL reagents on the market have been updated to include the new SM2.0 models (G400 FCL and G400 App-D series). The qualification switching is complete.
