G50 FAQs
G50 - Frequently Asked Questions
What is the product positioning of DNBSEQ-G50
DNBSEQ-G50 is a desktop, medium-to-low-throughput sequencer positioned as a compact, flexible, and efficient routine sequencing platform. Its throughput range is approximately 10-150 Gb, making it suitable for low-pass WGS, targeted gene sequencing, small-genome sequencing, transcriptome sequencing, metagenomic sequencing, and other applications.
Which customer types are most suitable for DNBSEQ-G50?
DNBSEQ-G50 is more suitable for customers who need a balanced combination of speed, throughput, and cost. It is especially suitable for customers with a certain level of batch sample testing demand who care more about overall cost performance than extreme turnaround speed. Recommended directions include reproductive health, pathogen detection, public health and disease control, and pathogen-related applications for industrial customers.
What are the core selling points of DNBSEQ-G50?
- Compact: desktop design with a footprint of less than 0.5 m².
- Flexible: supports both FCS and FCL flow cells, with read length up to PE150.
- Efficient: FCL SE50 can be shortened to approximately 7 hours, and full-load PE150 takes approximately 40 hours.
- Broad applications: covers pathogen detection, low-pass WGS, tumor panels, RNA sequencing, small-genome sequencing, and other scenarios.
What applications are recommended for DNBSEQ-G50?
- Small-genome sequencing, such as microbial metagenomics and single-bacterium sequencing
- Low-pass WGS, such as NIPT, PGS, and CNV analysis
- RNA sequencing, such as quantitative RNA and transcriptome sequencing
- Pathogen-related applications, including mNGS, tNGS, respiratory microorganisms, viral surveillance, and related applications
For microbial or small-genome sequencing, approximately how many samples can be run per run on DNBSEQ-G50?
- Metagenomic applications: ~20M reads/sample; FCL supports ~25 samples/run, FCS supports ~5 samples/run.
- Single-bacterium applications: ~1 Gb/sample; FCL supports ~100-150 samples/run, FCS supports ~20-23 samples/run.
For NIPT / PGS / CNV applications, approximately how many samples can be run per run on DNBSEQ-G50?
- NIPT / PGS: ~10M reads/sample; FCL supports ~50 samples/run, FCS supports ~10 samples/run.
- NIPT plus / CNV: ~25M reads/sample; FCL supports ~20 samples/run, FCS supports ~4 samples/run.
For RNA sequencing, approximately how many samples can be run per run on DNBSEQ-G50?
- Quantitative RNA: ~25M reads/sample; FCL supports ~20 samples/run, FCS supports ~4 samples/run.
- Transcriptome sequencing: ~6 Gb/sample; FCL supports ~25 samples/run, FCS supports ~5 samples/run.
What types of libraries does DNBSEQ-G50 support for sequencing?
The native sequencing workflow of DNBSEQ-G50 is based on DNB loading. For standard MGI libraries, circularization and DNB preparation must be completed before sequencing. For third-party libraries, it is recommended to use sequencing reagent sets with App models and complete the corresponding library conversion, circularization, and DNB preparation workflow.
Does DNBSEQ-G50 support third-party libraries for sequencing?
Yes, through the App-series library conversion solution, mainly for TruSeq and Nextera library types. Third-party libraries usually require two processing steps first: library adapter conversion (converting linear double-stranded DNA libraries into single-stranded circular DNA libraries compatible with the MGI platform) and sequencing primer replacement (selecting the appropriate App-A / App-C / App-D primers or reagent sets based on library type and sequencing mode). For DNBSEQ-G50, TruSeq libraries can use App-A; TruSeq/Nextera libraries can use App-C for PE sequencing; and TruSeq/Nextera/MGI libraries can use App-D, which supports single/dual barcode and SE/PE sequencing scenarios. Note that third-party library adapters remain third-party adapters, so downstream demultiplexing should be performed according to the third-party adapter information. For third-party libraries, customers should first confirm the library type, adapter system, whether conversion is required, and the compatible App-D reagents, as the App solution contains OS4.0 App reagent components and involves phosphorylation/conversion steps.
What App-D integrated reagent options are currently available for DNBSEQ-G50?
- 940-002167-00: DNBSEQ-G50RS High-throughput Sequencing Reagent Set App-D FCL SE50
- 940-002709-00: Sequencing Reaction Universal Reagent Kit, G50 PF-OS SM App-D FCL SE50
Does DNBSEQ-G50 support barcode import and hardware self-check?
Yes to both. Barcodes can be imported into the instrument from an external storage device, and the barcode is read later (not first) in the workflow. After startup, the system enters a self-check interface, and the maintenance interface also provides a self-check function that automatically performs hardware detection and displays the results. DNBSEQ-G50 does not support BBS.
What cleaning types are available for DNBSEQ-G50, and how long do they take?
- Regular cleaning: ~30 minutes; cleans residual reagents in the fluidic lines. Recommended before and after each sequencing run.
- Deep cleaning: ~45 minutes; cleans residual reagents and proteins in the fluidic lines to reduce blockage risk.
- Full cleaning: ~75 minutes; includes one regular cleaning and one deep cleaning. Required when the instrument hasn’t been cleaned for more than 7 days.
- Ultra-deep cleaning: ~12 hours; used for scheduled maintenance (e.g., first installation, upgrade installation, once every two weeks, or when not cleaned for more than 14 days) to reduce contamination and blockage risk.
Cleaning is done to remove residual reagents from the fluidic lines and flow cell stage, reducing the risk of cross-contamination, fluidic-line contamination, and blockage.
