T10x4 FAQs
T10X4 - Frequently Asked Questions
What is the DNBSEQ-T10×4RS and what is it used for?
The DNBSEQ-T10×4RS is an ultra-high-throughput sequencer based on DNBSEQ™ technology, designed for large-scale genome projects such as high-depth WGS and population cohorts ranging from hundreds of thousands to millions of samples.
What is the sequencing throughput of the T10×4RS?
A single system can run 8 slides simultaneously and generate around 20 Tb of data per day, supporting more than 50,000 30× WGS samples annually.
What read lengths does the T10×4RS support?
The system supports PE100 and PE150 modes:
- PE100: ~72 Tb output in ~96 hours
- PE150: ~76.8 Tb output in ~108 hours
How accurate is the sequencing data?
The platform delivers high accuracy with SNP accuracy ≥99.1% and indel accuracy ≥99%, alongside Q30 scores ≥85% and low duplication rates of around 1%.
What are the key advantages of the T10×4RS platform?
Its immersion-based open system improves reaction uniformity and allows reagent reuse, reducing costs. DNB technology also minimises error rates, duplication and index hopping while enabling high-density sequencing.
What is immersion sequencing and why does it matter?
Immersion sequencing ensures more uniform chemical reactions, avoids flow channel issues, reduces operating costs through reagent reuse, and supports stable high-throughput PE150 sequencing.
Can the T10×4RS be scaled for larger projects?
Yes. A single system supports ~50,000 WGS per year, and capacity can be scaled to 100,000–500,000+ genomes annually by adding additional systems and automation.
What are the main components of the system?
The system includes an optical detection unit, immersion reaction platform, high-density sequencing slides, DNB loading system, and integrated data processing via ZTRON or MegaBOLT.
What is the workflow time from DNA to FASTQ?
The full process takes approximately 4.5 days:
- ssCir preparation: ~8 hours
- DNB preparation: ~4 hours
- Sequencing: ~3.7 days
- FASTQ generation: ~8 hours (processed separately)
Does the T10×4RS support automation and different applications?
Yes. It integrates with automated systems for end-to-end workflows and supports multiple applications including PCR-free WGS, WES, RNA-seq, WGBS, stLFR, and single-cell sequencing.
