T20x2 FAQs
T20x2 - Frequently Asked Questions
What is T20×2?
T20×2 is an ultra-high-throughput sequencer with the highest production-scale throughput globally.
How does T20×2 achieve super-high sequencing throughput?
It uses large, high-density slides to load more samples per run and an open sequencing system supporting 6 slides and 2 imaging modules operating simultaneously to maximize efficiency.
Why does T20×2 deliver such low sequencing costs?
By employing dip-immersion biochemical reactions that allow reagent recycling (saving over 60% of reagents) and dual-color sequencing (saving 50% of optical, compute, and bandwidth resources compared to four-color systems).
What are T20×2’s throughput and recommended applications?
Single-slide throughput is 35B (PE100) or 40B (PE150), with 6 slides running concurrently. Recommended applications include WGS, WES, RNA-Seq, WGBS, stLFR, STOmics, ATAC, and single-cell RNA.
What distinguishes T20×2’s slide design from traditional flow cells?
T20×2 uses open slides immersed in reagent baths via robotic arms, eliminating flow-cell channels and glass coverslips to reduce costs and simplify fluidics and temperature control.
How does the open sequencing system differ from closed systems?
The open system offers greater space utilization for larger slides and more flexibility for future upgrades, unlike space-limited closed designs.
What advantages does dip-immersion biochemical reaction offer?
It recycles reagents across multiple slides, simplifies temperature control and fluidics, and ensures a stable, uniform reaction environment, unlike flow-mode systems where reagents become waste.
What bioinformatics capabilities does the BIT module provide?
Optional BIT module (via Ztron Pro) includes primary analysis (WFQ pipeline), secondary analysis (WGS/WES pipelines), customizable workflows, automated task management (ZLIMS Pro+), and an analysis application marketplace.
When and how is T20×2 being launched and delivered?
Launched at AGBT on February 7, 2023, with orders accepted immediately. China deliveries began in March 2023; APAC and Americas in H1 2023; Europe and Africa in H2 2023. Lead time is 3–6 months post-contract.
What are T20×2’s specifications and site requirements?
Without BIT module: weight 2743 kg; dimensions 4200 mm (L) × 4800 mm (W) × 2000 mm (H). Site load-bearing ≥ 500 kg/m² and area ≥ 37.2 m². Laboratory auxiliary equipment list available in the Installation Guide.
