DNBelab C Series scRNA Library Prep V3.0 FAQs
DNBelab C Series scRNA Library Prep V3.0 - Frequently Asked Questions
What is the DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0?
The DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) is a complete solution for single-cell transcriptome sequencing, enabling droplet generation, cell capture, and RNA library preparation in an integrated, high-throughput workflow.
What are the key features of this product?
The kit uses dual-bead cell capture technology to improve cell recognition accuracy and capture efficiency, achieves a multiplet contamination rate below 5% at 20,000 cells, incorporates a larger-diameter microfluidic tube to reduce clogging, and detects a median of over 2,000 genes per cell in standard samples such as PBMC and mouse brain nuclei.
Which instrument is required for droplet generation?
The V3.0 kit is compatible exclusively with the DNBelab C-TaiM 4 Single-cell Droplet Generator. The previous C4 device is not supported. The TaiM 4 must be operating under the “RNA V3” software strategy prior to use.
What sample types are supported?
The kit supports animal cells and nuclei, and plant protoplasts and nuclei. Validated species and tissue types include human, mouse, monkey, pig, and various plant species. Contact MGI technical support for guidance on unlisted or complex sample types.
What are the input requirements for cell or nucleus samples?
Cells or nuclei must have a diameter below 40 µm, a viability above 80%, and an impurity and aggregation rate below 5%. An input of fewer than 30,000 cells per run is recommended for optimal performance.
When should cell nuclei be used instead of whole cells?
Cell nuclei are recommended when working with samples stored at –80°C, clinically derived samples with long collection periods, tissues with low fresh dissociation success rates such as pancreatic tissue, or cells with irregular size and shape such as neurons.
How long does droplet generation take?
The droplet generator runs for up to 12 minutes, with generation typically completing between the 9th and 10th minute. The instrument stops automatically — no manual intervention is required.
Which sequencers and reagents are compatible?
The V3.0 kit is compatible with MGI sequencers including the DNBEQ-G400, DNBEQ-T7, and DNBEQ-T20, using PE100 or PE150 sequencing reagents. No change of sequencing primers is required. Note that the Oligo library cannot be pooling-sequenced with PE150 on the T20.
Can cDNA and Oligo libraries be pooled for sequencing?
Yes. When pooling by DNB mass, a cDNA to Oligo ratio of 15:1 is recommended as a starting point. Due to differences in fragment length, the actual sequencing output ratio will typically fall between 4:1 and 6:1 in favour of the cDNA library. Oligo libraries require a minimum of 50M reads regardless of cell input number.
How do I access the data analysis software?
The open-source analysis pipeline is available on GitHub under MGI-tech-bioinformatics/DNBelab_C_Series_HT_scRNA-analysis-software. Installation guides and usage documentation can also be downloaded from the MGI official website at mgi-tech.com/download/files. Demo data is available via the CNGB database at db.cngb.org.
