CycloneSEQ™ Library Preparation Kits
The CycloneSEQ™ Library Preparation Sets are specifically designed for library construction on the CycloneSEQ series sequencing platforms. Featuring high-quality enzymes, optimized buffers, and efficient purification reagents, these kits enable the preparation of genomic DNA (gDNA), amplicons, and other samples into high-quality libraries. From ultra-long reads to rapid 7-minute prep and high-throughput 96-plex multiplexing, the series delivers flexibility, efficiency, and superior data performance.
Product
| Product Name | Catalog Number | Specification |
|---|---|---|
| CycloneSEQ Ultra-long Library Prep Set S1 | H940-000153 | 6 RXN |
| CycloneSEQ Rapid 24 Barcode Library Prep Set | H940-000099 | 6 RXN |
| CycloneSEQ 96 Barcode Library Prep Set S1 | H940-000118 | 3 RXN |
| CycloneSEQ 24 Barcode Library Prep Set S1 | H940-000108 | 4 RXN |
| CycloneSEQ Universal Library Prep Set S1 | H940-000107 | 24 RXN |
| CycloneSEQ Barcode Sequencing Adaptor S1 | H940-000117 | 4 RXN |
Product Specifications & Working Principles
CycloneSEQ Ultra-long Library Prep Set S1
| Description | Suitable for constructing ultra-long read libraries from high-molecular-weight (HMW) genomic DNA of various species (human, animal, plant). Requires dedicated ultra-long sequencing mode and CycloneSEQ G400-ER Sequencing Flow Cell S1. |
|---|---|
| Working Principle | Processes genomic double-stranded DNA (dsDNA) through end repair, sequencing adaptor ligation, and precipitation-based purification to construct sequencing-ready libraries. |
| Specification | 6 RXN |
| Storage Temperature | Box 1: -25℃ ~ -15℃ Box 2: 2℃ ~ 8℃ |
CycloneSEQ Rapid 24 Barcode Library Prep Set
| Description | Specifically designed for DNA amplification products. Completes single-sample library prep in just 7 minutes, significantly improving efficiency. |
|---|---|
| Working Principle | Processes PCR-amplified DNA products through end repair, barcode and sequencing adaptor ligation, and bead purification. |
| Specification | 6 RXN |
| Storage Temperature | Box 1 & 2: -25℃ ~ -15℃ Box 3: 2℃ ~ 8℃ |
CycloneSEQ 96 Barcode Library Prep Set S1
| Description | Designed for library construction allowing pooling and sequencing of up to 96 different samples. Compatible with gDNA, amplicons, and microbial gDNA. |
|---|---|
| Working Principle | Constructs sequencing libraries through end repair, barcode ligation, sequencing adaptor ligation, and beads-based purification. |
| Specification | 3 RXN |
| Storage Temperature | Box 1 & 2: -25℃ ~ -15℃ Box 3: 2℃ ~ 8℃ |
CycloneSEQ 24 Barcode Library Prep Set S1
| Description | Designed for library construction allowing pooling and sequencing of up to 24 different samples. Compatible with gDNA, amplicons, and microbial gDNA. |
|---|---|
| Working Principle | Constructs sequencing libraries through end repair, barcode ligation, sequencing adaptor ligation, and beads-based purification. |
| Specification | 4 RXN |
| Storage Temperature | Box 1: -25℃ ~ -15℃ Box 2: 2℃ ~ 8℃ |
CycloneSEQ Universal Library Prep Set S1
| Description | Preferred enzymatic ligation system significantly improves ligation efficiency for long fragments. Compatible with CycloneSEQ series platforms. |
|---|---|
| Working Principle | Constructs libraries through ligation of barcodes and adaptors and beads-based purification. |
| Specification | 24 RXN |
| Storage Temperature | Box 1: -25℃ ~ -15℃ Box 2: 2℃ ~ 8℃ |
CycloneSEQ Barcode Sequencing Adaptor S1
| Description | Contains barcoded sequencing adapters identical in composition to those in the 24 and 96 Barcode Library Preparation Sets, serving as compatible supplements. |
|---|---|
| Specification | 4 RXN |
| Storage Temperature | -25℃ ~ -15℃ |
Highlights
Ultra-Long Read Capability
- Optimized for high-molecular-weight (HMW) genomic DNA, enabling the construction of ultra-long read libraries for advanced genomic assembly.
Rapid & Efficient Workflow
- The Rapid 24 Barcode Set completes single-sample library preparation in just 7 minutes, drastically reducing turnaround time.
High-Throughput Multiplexing
- Flexible barcode options (24 or 96) allow for efficient pooling and sequencing of multiple samples, maximizing platform throughput.
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