MGIEasy Duplex UMI Universal Library Prep Set

High-throughput sequencing is increasingly essential for cancer screening and drug selection, particularly for detecting low-frequency variants. Duplex-UMI (unique molecule identifiers) provide enhanced sensitivity and precision, making them a critical tool for accurate sequencing. The MGIEasy Exome Universal Library Prep Set supports 10–1000 ng DNA input and uses duplex barcodes to minimize label hopping and sample cross-contamination. UMIs mark original molecules, allowing error correction to reduce PCR and sequencing artifacts.

Optimized with high-quality enzymes, improved adapter ligation, and high-fidelity polymerases, this kit significantly boosts library conversion and amplification efficiency. It is compatible with FFPE and low-input cfDNA, providing a reliable, high-precision solution for exome library construction in cancer research and other applications requiring sensitive variant detection.

Product

Product Name Item Number Component Component Item Number
MGIEasy Duplex UMI Universal Library Prep Set (16 RXN) 1000018643 Library: MGIEasy Universal DNA Library Prep Module 1000019376
Adapters: MGIEasy Duplex UMI Adapters Kit 1000018645
Clean Beads: MGIEasy DNA Clean Beads 1000005278
Circularization: MGIEasy Dual Barcode Circularization Module 1000018649
MGIEasy Duplex UMI Universal Library Prep Set (96 RXN) 1000018644 Library: MGIEasy Universal DNA Library Prep Module 1000019377
Adapters: MGIEasy Duplex UMI Adapters Kit 1000018646
Clean Beads: MGIEasy DNA Clean Beads 1000005279
Circularization: MGIEasy Dual Barcode Circularization Module 1000018649

Product Specification

ProductMGIEasy Duplex UMI Universal Library Prep Set
LotV1.0
Version16 RXN (1000008643) & 96 RXN (1000018644)
Fragmentation methodUltrasound except cfDNA
Library size200 – 600 bp
Assay time~7 hrs
Hands-on time~30 mins
Sample requirementsHuman genome DNA, including Blood, tissue, FFPE, cfDNA
Suitable Hybridization ProbesMGI, Agilent, Nimblegen, IDT or similar probes from other vendors
Sequencing PlatformDNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7
Read lengthPE100, PE150

Recommended Workflow

ApplicationsLibrary preparationSequencingAnalysis
Low frequency variant detection
  • MGISP-100
  • MGISP-960
  • MGIEasy Exome Universal Library Prep Set
  • MGI, Agilent, Nimblegen, IDT or similar probes from other vendors
  • DNBSEQ-G50* (PE100, PE150)
  • DNBSEQ-G400* (PE100, PE150)
  • DNBSEQ-T7* (PE100, PE150)
UMI analysis package (UAP)

Highlights

Minimize Sample Contamination

  • Duplex barcodes reduce barcode swapping and minimize contamination between samples for more reliable sequencing results.

High Conversion Efficiency

  • Supports library construction from as little as 10 ng DNA, thanks to a high library conversion rate.

Ultra-Low Frequency Variant Detection

  • Over 200 UMIs enable sensitive detection of ultra-low frequency variants, enhancing precision for cancer and genetic research.

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