MGIEasy Duplex UMI Universal Library Prep Set
High-throughput sequencing is increasingly essential for cancer screening and drug selection, particularly for detecting low-frequency variants. Duplex-UMI (unique molecule identifiers) provide enhanced sensitivity and precision, making them a critical tool for accurate sequencing. The MGIEasy Exome Universal Library Prep Set supports 10–1000 ng DNA input and uses duplex barcodes to minimize label hopping and sample cross-contamination. UMIs mark original molecules, allowing error correction to reduce PCR and sequencing artifacts.
Optimized with high-quality enzymes, improved adapter ligation, and high-fidelity polymerases, this kit significantly boosts library conversion and amplification efficiency. It is compatible with FFPE and low-input cfDNA, providing a reliable, high-precision solution for exome library construction in cancer research and other applications requiring sensitive variant detection.
Product
| Product Name | Item Number | Component | Component Item Number |
|---|---|---|---|
| MGIEasy Duplex UMI Universal Library Prep Set (16 RXN) | 1000018643 | Library: MGIEasy Universal DNA Library Prep Module | 1000019376 |
| Adapters: MGIEasy Duplex UMI Adapters Kit | 1000018645 | ||
| Clean Beads: MGIEasy DNA Clean Beads | 1000005278 | ||
| Circularization: MGIEasy Dual Barcode Circularization Module | 1000018649 | ||
| MGIEasy Duplex UMI Universal Library Prep Set (96 RXN) | 1000018644 | Library: MGIEasy Universal DNA Library Prep Module | 1000019377 |
| Adapters: MGIEasy Duplex UMI Adapters Kit | 1000018646 | ||
| Clean Beads: MGIEasy DNA Clean Beads | 1000005279 | ||
| Circularization: MGIEasy Dual Barcode Circularization Module | 1000018649 |
Product Specification
| Product | MGIEasy Duplex UMI Universal Library Prep Set |
| Lot | V1.0 |
| Version | 16 RXN (1000008643) & 96 RXN (1000018644) |
| Fragmentation method | Ultrasound except cfDNA |
| Library size | 200 – 600 bp |
| Assay time | ~7 hrs |
| Hands-on time | ~30 mins |
| Sample requirements | Human genome DNA, including Blood, tissue, FFPE, cfDNA |
| Suitable Hybridization Probes | MGI, Agilent, Nimblegen, IDT or similar probes from other vendors |
| Sequencing Platform | DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7 |
| Read length | PE100, PE150 |
Recommended Workflow
| Applications | Library preparation | Sequencing | Analysis |
|---|---|---|---|
| Low frequency variant detection |
|
| UMI analysis package (UAP) |
Highlights
Minimize Sample Contamination
- Duplex barcodes reduce barcode swapping and minimize contamination between samples for more reliable sequencing results.
High Conversion Efficiency
- Supports library construction from as little as 10 ng DNA, thanks to a high library conversion rate.
Ultra-Low Frequency Variant Detection
- Over 200 UMIs enable sensitive detection of ultra-low frequency variants, enhancing precision for cancer and genetic research.
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