MGIEasy Exome Capture V5 Probe Set

The MGIEasy Exome Capture V5 Probe Set provides comprehensive coverage beyond traditional exome probes, targeting coding sequences associated with a wide range of diseases, including reproductive, neonatal, cardiovascular, cerebrovascular, hereditary tumours, deafness, monogenic disorders, medication safety, immunodeficiency, personal genome analysis, and mitochondrial defects. With a design size of 69 Mb, the primary targets include CCDS, GENCODE, RefSeq, miRBase, and the MGI Clinical Database.

As a universal exome capture solution, this probe set is compatible with MGI and other NGS platforms, making it an ideal choice for researchers seeking high-quality, clinically relevant exome sequencing.

Product

Product Name	Item Number	Component	Component Item Number
MGIEasy Exome Capture V5 Probe Set (16 RXN)	940-000187-00	Probes: MGIEasy Exome Capture V5 Probe (16 RXN)	1000007741
		Hybridization and elution: MGIEasy Exome Capture Hybridization and Wash Kit (Box 1) (16 RXN)	940-000168-00
		Hybridization and elution: MGIEasy Exome Capture Hybridization and Wash Kit (Box 2) (16 RXN)	940-000169-00

Product Specification

Kit name	MGIEasy Exome Capture V5 Probe Set
Probe length	90nt
Covered region size	69 Mb
Databases used for probe design	CCDS, GENCODE, RefSeq, miRBase, MGI Clinical Database
Covered regions	Traditional exome regions and various disease-related regions
Storage	-80℃, -25℃ to -18℃, Room temperature (refer to kit label)
DNA input	1 μg PCR product per sample for single hybridization; >250 ng each sample for multiplex; total 1–2 μg
Number of samples per hybridization	1-8
Sample types	PCR product
Compatible species	Human
Compatible sequencing platforms	Platforms from MGI, Illumina, and Thermo Fisher
Read length	PE100/PE150
Detectable mutation types	SNV & Indel
Technologies	Capture + NGS
Recommended Probes for Capture	MGIEasy Exome Capture V5 Probe Set
Recommended Library Preparation Sets	MGIEasy Exome FS Library Prep Set (1000009658), MGIEasy Exome Universal Library Prep Set, Other library preparation kits
Compatible Sequencing Platforms	DNBSEQ-T7, DNBSEQ-G400
Recommended Applications	Whole exome sequencing, Target sequencing

Recommended Workflow

Applications	Library preparation	Sequencing	Bioinformation analysis
Whole exome sequencing	MGIEasy Exome Universal Library Prep Set MGIEasy Exome FS Library Prep Set MGIEasy Exome Capture V5 Probe Set	DNBSEQ-G400*

Highlights

Comprehensive 69 Mb Probe Coverage

Comprehensive 69 Mb coverage: Designed from CCDS, GENCODE, RefSeq, miRBase, and other databases.
Disease-relevant targeting: Ensures capture of coding sequences related to reproductive, neonatal, cardiovascular, cerebrovascular, hereditary tumors/deafness, monogenic disorders, medication safety, personal genome, immunodeficiency, and mitochondrial defects.