MGIEasy Fast FS Library Prep Set V2.0

The MGIEasy Fast FS Library Prep Set V2.0 streamlines genomic DNA (gDNA) library preparation for DNBSEQ™ sequencers by combining fragmentation, end-repair, and A-tailing into a single step. This simplified workflow reduces prep time to just 2 hours, making high-quality library construction faster and easier.

With flexible input ranging from 1 ng to 1 μg, the kit supports a wide variety of sample types, including blood, saliva, oral swabs, FFPE, and other precious low-input samples. It’s ideal for microbial sequencing, metagenomics, whole-genome sequencing (WGS), and whole-exome sequencing (WES), providing a reliable, efficient solution for diverse research applications.

Product

MGIEasy Fast FS Library Prep Set V2.0 (16 RXN) (Without circularization reagent)
Item No.: 940-002915-00

Component Kits Component Kits Name & Item No. Quantity
Core library MGIEasy Fast FS Library Prep Module V2.0 (16 RXN)
Item No.: 940-001197-00
1
PCR primers & Adapter MGIEasy UDB Primers Adapter Kit (16 barcode, 16 RXN)
Item No.: 940-001764-00
1
Clean Beads MGIEasy DNA Clean Beads (3.2 mL beads)
Item No.: 940-001176-00
1

MGIEasy Fast FS Library Prep Set B V2.0 (96 RXN) (Without circularization reagent)
Item No.: 940-002917-00

Component Kits Component Kits Name & Item No. Quantity
Core library MGIEasy Fast FS Library Prep Module V2.0 (96 RXN)
Item No.: 940-001195-00
1
PCR primers & Adapter MGIEasy UDB Primers Adapter Kit B (96 barcode, 96 RXN)
Item No.: 940-001762-00
1
Clean Beads MGIEasy DNA Clean Beads (15 mL beads)
Item No.: 940-001174-00
1

MGIEasy Fast FS Library Prep Set C V2.0 (96 RXN) (Without circularization reagent)
Item No.: 940-001831-00

Component Kits Component Kits Name & Item No. Quantity
Core library MGIEasy Fast FS Library Prep Module V2.0 (96 RXN)
Item No.: 940-001195-00
1
PCR primers & Adapter MGIEasy UDB Primers Adapter Kit C (96 barcode, 96 RXN)
Item No.: 940-001748-00
1
Clean Beads MGIEasy DNA Clean Beads (15 mL beads)
Item No.: 940-001174-00
1

MGIEasy Fast FS Library Prep Set A V2.0 (96 RXN) (Without circularization reagent)
Item No.: 940-002916-00

Component Kits Component Kits Name & Item No. Quantity
Core library MGIEasy Fast FS Library Prep Module V2.0 (96 RXN)
Item No.: 940-001195-00
1
PCR primers & Adapter MGIEasy UDB Primers Adapter Kit A (96 barcode, 96 RXN)
Item No.: 940-001763-00
1
Clean Beads MGIEasy DNA Clean Beads (15 mL beads)
Item No.: 940-001174-00
1

Product Specification

 
Version V2.0
Assay Time ~2 hrs (from gDNA to PCR library)
Input Quantity 1 ng ~ 200 ng gDNA for small genomes (e.g. microbial, meta), 25 ng ~ 1 μg gDNA for large genomes (e.g. human, plant, animal)
Barcode ~288 Unique Dual Barcodes
Sample types gDNA from blood, saliva, oral swabs, tissue, Microbial gDNA, Meta DNA, FFPE DNA
Species Compatibility Human, Animal, Plant, Microbe
Applications Human WGS, Plants & Animals WGS, Microbial WGS, Metagenomics sequencing, WES
Platform Compatibility DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-G99, DNBSEQ-E25, DNBSEQ-T7, DNBSEQ-T10×4, DNBSEQ-T20×2
Recommended Read Length SE100 / PE100 / PE150
Adaptation automation platform MGISP-100 (16 RXN), MGISP-960 (96 RXN)

Recommended Workflow

ApplicationsLibrary preparationSequencingData analysis
  • Metagenomics sequencing
  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0
  • DNBSEQ OneStep DNB Make Reagent Kit V2.0 (OS-DB) (940-000036-00)
  • DNBSEQ-G99
  • MGISEQ-G50
  • DNBSEQ-E25
  • MGISEQ-G400
  • DNBSEQ-T7
  • DNBSEQ-T10×4
  • Platform of microorganisms Fast Identification (PFI)
  • Platform of microorganisms Fast Identification (PFI)
  • Human WGS
  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0
  • MGIEasy Dual Barcode Circularization Kit
  • MGISEQ-G400
  • DNBSEQ-T7
  • DNBSEQ-T10×4
  • DNBSEQ-T20×2
  • MegaBOLT Bioinformatics Analysis Accelerator
  • WGS Germline variant calling pipeline
  • WGS Somatic variant calling pipeline
  • Human WES
  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0
  • MGIEasy Dual Barcode Exome Capture Accessory Kit
  • MGIEasy Exome Capture V5 Probe Set or other commercialised WES probes
  • MGIEasy Dual Barcode Circularization Kit
  • MGISEQ-G400
  • DNBSEQ-T7
  • MegaBOLT Bioinformatics Analysis Accelerator
  • WES Germline variant calling pipeline
  • WES Somatic variant calling pipeline
  • Plant/Animal WGS (For Plant/Animal molecule breeding, population evolution, etc.)
  • MGISP-100/MGISP-960
  • MGIEasy Fast FS Library Prep Set V2.0
  • MGIEasy Dual Barcode Circularization Kit
  • MGISEQ-G400
  • DNBSEQ-T7
  • DNBSEQ-T10×4

Highlights

Fast Library Prep

  • Combines DNA fragmentation, end-repair, and A-tailing in a single step, reducing library preparation time to just 2 hours..

Flexible Workflow

  • Supports 1 ng–1 μg DNA input and a variety of sample types—blood, saliva, oral swabs, FFPE, and low-input precious samples—suitable for microbial, metagenomics, WGS, and WES applications.

Consistent Insert Sizes

  • High-quality, low-bias enzymes deliver uniform and reliable insert sizes across a wide range of DNA inputs and sample types for reproducible results.

Automatable Process

  • Compatible with MGISP-100 and MGISP-960 systems, enabling fully automated library preparation workflows to boost efficiency and throughput.

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