MGIEasy Fast Hybridization and Wash Kit
The MGIEasy Fast Hybridization and Wash Kit is designed for rapid, high-quality targeted DNA capture. It includes hybridization elution reagents and streptavidin magnetic beads to simplify and standardize the hybridization process for DNA probes. With broad compatibility and optimized protocols, the kit reduces hybridization time to under 2 hours without compromising data quality, significantly shortening liquid-phase probe capture workflows.
When paired with the MGIEasy library prep series, it supports construction and targeted enrichment of exome and panel libraries on MGI high-throughput sequencing platforms, offering a fast, reliable solution for researchers performing high-throughput targeted sequencing projects.
Product
| Product Name | Item Number | Component Kit | Component Kit Part Number | Quantity in Set |
|---|---|---|---|---|
| MGIEasy Fast Hybridization and Wash Kit (96 RXN) | 940-001973-00 | Hybridization elution reagents (BOX 1) | 940-001826-00 | 1 |
| Streptavidin magnetic beads (BOX 2) | 940-001828-00 | 1 | ||
| MGIEasy Fast Hybridization and Wash Kit (16 RXN) | 940-001974-00 | Hybridization elution reagents (BOX 1) | 940-001825-00 | 1 |
| Streptavidin magnetic beads (BOX 2) | 940-001823-00 | 1 |
Product Specification
| Specification | Details |
|---|---|
| Applications | Hybridization capture of target regions in human samples, such as WES (Whole Exome Sequencing), small panel sequencing |
| Probes | Commercial DNA probes (e.g., IDT, Twist, Nanod, BOKE) |
| Hybridization time | 0.5h to 24h |
| Sample type | Human gDNA, FFPE DNA, cfDNA |
| Platform Compatibility | MGISEQ-2000, DNBSEQ-G99, DNBSEQ-T7 |
| Pre-library | Pre-PCR library prepared using MGI double barcode adapters for hybridization reaction. Recommended fragment size: 200–300 bp main band distribution |
| Multi-plex hybridization | Compatible with single-plex and multi-plex samples hybridization (supports up to 12 samples) |
| Circularization | MGI universal circularization |
| Recommended Applications |
|
| Applications | Whole exon sequencing / target sequencing |
Recommended Workflow
| Applications | Library preparation | Sequencing | Data analysis |
|---|---|---|---|
|
|
|
|
|
|
|
Highlights
Fast Hybridization
- Reduces hybridization time to under 2 hours while maintaining high data quality. Combined with the MGIEasy Fast FS Library Prep Set V2.0, gDNA to enriched libraries can be completed in one day.
Flexible Hybridization
- Offers 0.5–2 hour fast hybridization or traditional overnight protocols, allowing researchers to choose based on workflow needs.
Broad Probe Compatibility
- Works with multiple DNA probes, including IDT, Twist, Nanod, and BOKE, for versatile targeted capture applications.
Enhanced Performance
- Delivers high post-capture yield, low duplication rates, and uniform coverage, ensuring reliable and reproducible enrichment results.
Discover More
Get in Touch
Phone
📞(+86) 4000-688-114
Marketing & PR
MGI_Branding@mgi-tech.com
Careers
MGI_Recruiting@mgi-tech.com
Investor Relations
MGI_IR@mgi-tech.com
