MGIEasy Fast PCR-FREE FS Library Prep Set V2.0

The MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 enables fast, PCR-free whole genome library preparation for DNBSEQ™ sequencers. Optimized for 25 ng–900 ng genomic DNA, it combines fragmentation, end-repair, and A-tailing in a single step, simplifying workflows and significantly reducing prep time. Truly PCR-free sequencing ensures no amplification errors, while optimized protocols deliver uniform genome coverage and high precision in mutation detection. Compatible with MGISP-100 and MGISP-960 systems, it supports automated library preparation for higher throughput.

Ideal for large-scale WGS projects, this kit provides a fast, reliable, and easy-to-use solution, making it the preferred choice for researchers seeking high-quality, PCR-free sequencing results.

Product

MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 (16 RXN)
Item No.: 940-000886-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast PCR-FREE FS Library Prep Module V2.0 (16 RXN) Item No.: 940-000885-00	1
Adapter	MGIEasy UDB PF Adapter Kit (16 barcode, 16 RXN) Item No.: 940-000018-00	1
Clean Beads	MGIEasy DNA Clean Beads (3.2 mL beads) Item No.: 940-001176-00	1

MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 (96 RXN)
Item No.: 940-000884-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast PCR-FREE FS Library Prep Module V2.0 (96 RXN) Item No.: 940-000883-00	1
Adapter	MGIEasy UDB PF Adapter Kit A (96 barcode, 96 RXN) Item No.: 940-000023-00	1
Clean Beads	MGIEasy DNA Clean Beads (15 mL beads) Item No.: 940-001174-00	1

MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 (384 RXN)
Item No.: 940-000882-00

Component Kits	Component Kits Name & Item No.	Quantity
Core library	MGIEasy Fast PCR-FREE FS Library Prep Module V2.0 (96 RXN) Item No.: 940-000883-00	4
Adapter	MGIEasy UDB PF Adapter Kit A (96 barcode, 96 RXN) Item No.: 940-000023-00	1
	MGIEasy UDB PF Adapter Kit B (96 barcode, 96 RXN) Item No.: 940-000022-00	1
	MGIEasy UDB PF Adapter Kit C (96 barcode, 96 RXN) Item No.: 940-000025-00	1
	MGIEasy UDB PF Adapter Kit D (96 barcode, 96 RXN) Item No.: 940-000024-00	1
Clean Beads	MGIEasy DNA Clean Beads (50 mL beads) Item No.: 1000005279	1

Product Specification

Version	V2.0
Assay Time	~1.3 hrs – 1.4 hrs
Hands-On Time	~20 min
Input Quantity	25 ng ~ 900 ng gDNA
Barcode	~384 Unique Dual Barcodes
Insert size	Two sides beads purification: 400 bp ~ 600 bp One side beads purification: 600 bp ~ 850 bp
Sample types	gDNA from blood, saliva, oral swabs, tissue, Microbial gDNA, Meta DNA, Virus long amplicons
Species Compatibility	Human, Animal, Plant, Microbe
Applications	Human WGS, Plants & animals WGS, Low-pass WGS, Microbial WGS, Metagenomics sequencing, Virus long amplicons sequencing
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7, DNBSEQ-T10×4, DNBSEQ-T20×2, DNBSEQ-G99, DNBSEQ-E25
Recommended Read Length	SE100 / PE100 / PE150
Adaptation automation platform	MGISP-100 (16 RXN), MGISP-960 (96 RXN, 384 RXN)

Recommended Workflow

Applications	Library preparation	Sequencing	Data analysis
Human WGS sequencing (High depth)	MGISP-100/MGISP-960 MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-T7 DNBSEQ-T10×4 DNBSEQ-T20×2	MegaBOLT Bioinformatics Analysis Accelerator WGS Germline variant calling pipeline WGS Somatic variant calling pipeline
Low-pass WGS	MGISP-960 MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 MGIEasy Dual Barcode Circularization Kit	DNBSEQ-G400 DNBSEQ-T7

Highlights

Fast Library Prep

Combines fragmentation, end-repair, and A-tailing in a single step, completing library preparation in just 1.3 hours for rapid WGS workflows.

Wide Sample Compatibility

Handles 25 ng–900 ng DNA from blood, saliva, oral swabs, or viral amplicons. Suitable for high/low-depth WGS, microbial sequencing, and pathogen amplicon detection.

High Uniformity

Uses low-bias, high-quality enzymes to produce consistent insert sizes and uniform coverage across DNA inputs and species for reliable sequencing results..

PCR-Free Accuracy & Automation

Truly PCR-free, eliminating amplification errors. Minimal hands-on steps and compatibility with MGISP-100/960 enables fully automated library prep for precision and efficiency.