MGIEasy FS PCR-Free DNA Library Prep Set
The MGIEasy FS PCR-Free DNA Library Prep Set is designed for constructing WGS libraries without PCR amplification on MGI high-throughput sequencing platforms. Using a high-quality, low-bias fragmentation enzyme, the kit efficiently fragments 50–1000 ng of gDNA with a size distribution comparable to mechanical fragmentation methods.
When paired with the MGISP automatic sample preparation system, the kit enables a fully automated library preparation workflow, reducing hands-on time and variability. Combined with the MGI DNBSEQ™ sequencer, this workflow provides true PCR-free sequencing, eliminating amplification errors and delivering high-fidelity whole-genome sequencing data. Ideal for laboratories seeking accurate, reproducible, and streamlined WGS library preparation, the MGIEasy FS PCR-Free DNA Library Prep Set ensures consistent performance across a wide range of genomic samples.
Product
| Product Name | Item Number | Component | Component Item Number |
|---|---|---|---|
| MGIEasy FS PCR-Free DNA Library Prep Set (96 RXN) | 1000013455 | Library and Circularization: MGIEasy FS PCR-Free DNA Library Prep Kit | 1000013459 |
| Adapter: MGIEasy PF Adapters-96 (plate) Kit | 1000013461 | ||
| Beads: MGIEasy DNA Clean Beads (50 mL) | 940-001594-00 | ||
| MGIEasy FS PCR-Free DNA Library Prep Set (16 RXN) | 1000013454 | Library and Circularization: MGIEasy FS PCR-Free DNA Library Prep Kit | 1000013458 |
| Adapter: MGIEasy PF Adapters-16 (tube) Kit | 1000013460 | ||
| Beads: MGIEasy DNA Clean Beads (8 mL) | 940-001596-00 |
Product Specification
| Product Name | MGIEasy FS PCR-Free DNA Library Prep Set |
| Application | Whole genome sequencing |
| Version | V1.2 (16 RXN), V1.3 (96 RXN) |
| Configuration (Cat.) | 16 RXN (1000013454), 96 RXN (1000013455) |
| Validity period | 9 months |
| Assay Time | ~3.9 hrs |
| Input Quantity | 50-1000 ng gDNA |
| Sample types | gDNA, WGA DNA |
| Species Compatibility | Human, animals, plants, fungi, bacteria, metagenomics, etc. |
| Fragmentation Method | Enzymatic |
| Recommended Insert Size | 350-660 bp |
| Platform Compatibility | DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7 |
| Recommended Read Length | SE100, PE100, PE150 |
Recommended Workflow
| Application | Library Preparation | Sequencing | Analysis |
|---|---|---|---|
| Human WGS sequencing (High depth) |
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Highlights
Broad Species Compatibility
- High-quality, low-bias enzyme fragments DNA from different species and input amounts consistently under the same incubation conditions.
Stable Library Yield
- Modified reaction system ensures high-quality libraries with consistent and reliable yields across samples.
Simple and Automated Workflow
- Complete library construction in 3.9 hours with compatibility for automatic sample preparation systems, streamlining your workflow.
No Amplification Errors
- True PCR-free WGS on MGI DNBSEQ™ platforms eliminates PCR amplification errors, delivering high-fidelity genome sequencing.
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