MGIEasy FS PCR-Free DNA Library Prep Set

The MGIEasy FS PCR-Free DNA Library Prep Set is designed for constructing WGS libraries without PCR amplification on MGI high-throughput sequencing platforms. Using a high-quality, low-bias fragmentation enzyme, the kit efficiently fragments 50–1000 ng of gDNA with a size distribution comparable to mechanical fragmentation methods.

When paired with the MGISP automatic sample preparation system, the kit enables a fully automated library preparation workflow, reducing hands-on time and variability. Combined with the MGI DNBSEQ™ sequencer, this workflow provides true PCR-free sequencing, eliminating amplification errors and delivering high-fidelity whole-genome sequencing data. Ideal for laboratories seeking accurate, reproducible, and streamlined WGS library preparation, the MGIEasy FS PCR-Free DNA Library Prep Set ensures consistent performance across a wide range of genomic samples.

Product

Product Name Item Number Component Component Item Number
MGIEasy FS PCR-Free DNA Library Prep Set (96 RXN) 1000013455 Library and Circularization: MGIEasy FS PCR-Free DNA Library Prep Kit 1000013459
Adapter: MGIEasy PF Adapters-96 (plate) Kit 1000013461
Beads: MGIEasy DNA Clean Beads (50 mL) 940-001594-00
MGIEasy FS PCR-Free DNA Library Prep Set (16 RXN) 1000013454 Library and Circularization: MGIEasy FS PCR-Free DNA Library Prep Kit 1000013458
Adapter: MGIEasy PF Adapters-16 (tube) Kit 1000013460
Beads: MGIEasy DNA Clean Beads (8 mL) 940-001596-00

Product Specification

Product NameMGIEasy FS PCR-Free DNA Library Prep Set
ApplicationWhole genome sequencing
VersionV1.2 (16 RXN), V1.3 (96 RXN)
Configuration (Cat.)16 RXN (1000013454), 96 RXN (1000013455)
Validity period9 months
Assay Time~3.9 hrs
Input Quantity50-1000 ng gDNA
Sample typesgDNA, WGA DNA
Species CompatibilityHuman, animals, plants, fungi, bacteria, metagenomics, etc.
Fragmentation MethodEnzymatic
Recommended Insert Size350-660 bp
Platform CompatibilityDNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7
Recommended Read LengthSE100, PE100, PE150

Recommended Workflow

ApplicationLibrary PreparationSequencingAnalysis
Human WGS sequencing (High depth)
  • MGIEasy FS PCR-Free DNA Library Prep Set
  • MGISP-100
  • MGISP-960
  • DNBSEQ-G400*
  • DNBSEQ-G50*
  • DNBSEQ-T7*
  • MegaBOLT Bioinformatics analysis accelerator
  • WGS germline mutation analysis software
  • WGS somatic mutation analysis software

Highlights

Broad Species Compatibility

  • High-quality, low-bias enzyme fragments DNA from different species and input amounts consistently under the same incubation conditions.

Stable Library Yield

  • Modified reaction system ensures high-quality libraries with consistent and reliable yields across samples.

Simple and Automated Workflow

  • Complete library construction in 3.9 hours with compatibility for automatic sample preparation systems, streamlining your workflow.

No Amplification Errors

  • True PCR-free WGS on MGI DNBSEQ™ platforms eliminates PCR amplification errors, delivering high-fidelity genome sequencing.

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