MGIEasy Pa-SNPs Genotyping Kit
The MGIEasy Pa-SNPs Genotyping Kit enables rapid detection of 2010 high-frequency human SNPs. Built on MGI’s proprietary ATOPlex multiplex PCR platform, the kit provides all reagents needed to prepare sequencing libraries from DNA samples.
It is compatible with a wide range of sample types, including cfDNA from plasma, gDNA from blood, saliva, tissue, and sperm. Specifically designed for forensic genomics applications, the kit supports non-invasive prenatal paternity testing, standard paternity testing, and individual identification with high accuracy and reliability. Ideal for laboratories requiring fast, high-throughput, and versatile SNP genotyping, the MGIEasy Pa-SNPs Genotyping Kit streamlines library preparation while maintaining robust performance across diverse sample types.
Product
| Product Name | Item Number | Box | Contents |
|---|---|---|---|
| MGIEasy Pa-SNPs Genotyping Kit (96 RXN) | 1000016270 | Box 1 | PCR primer, PCR enzyme, buffer, circularization reagents, digestion reagents, anti-contamination reagents |
| Box 2 | 96 PCR barcode primer | ||
| Box 3 | Two types of beads for PCR product cleanup and digestion product cleanup |
Product Specification
| Product name | MGIEasy Pa-SNPs Genotyping Kit |
| Version | V1.0 |
| Reaction/kit | 96 RXN/kit |
| Catalog No. | 1000016270 |
| Species Compatibility | Human |
| Sample types | cfDNA from plasma; gDNA from blood, tissues, blood spot, saliva, buccal swabs, semen, hair, and nails |
| Recommended Input Quantity | cfDNA: 8–20 ng, DNA concentration ≥0.4 ng/µL; gDNA: 1–20 ng |
| Library Method | Multiplex PCR |
| Amplicon Size | 60–90 bp |
| Library Time | 6.5 hrs |
| Recommended Read Length | SE50 |
| Platform Compatibility | DNBSEQ-G400, DNBSEQ-G50 |
| Recommended sequencing data | cfDNA: ≥25M reads; gDNA: ≥5M reads |
Recommended Workflow
| Applications | Library preparation | Sequencing | Bioinformation analysis |
|---|---|---|---|
| Non-invasive prenatal paternity testing | MGIEasy Pa-SNPs Genotyping Kit |
|
Highlights
Easy to Use
- Two-step PCR in a single tube enables amplification of 2010 SNPs with minimal handling..
Fast and Efficient Workflow
- Complete the workflow in 6.5 hours total with only 1.5 hours of hands-on time.
High Sensitivity
- Detect SNPs from as little as 1 ng of DNA, ensuring reliable results from low-input samples.
High Accuracy
- Accurately detects minor contributors as low as 2%, making it suitable for precise forensic analysis.
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