MGIEasy PCR-Free DNA Library Prep Set

The MGIEasy PCR-Free Library Prep Set is designed for whole-genome sequencing (WGS) library construction on MGI high-throughput sequencing platforms. Optimized for 80–200 ng of size-selected fragmented DNA, it converts DNA into high-quality, customized libraries.

Combined with the MGI DNBSEQ™ sequencer, this true PCR-free workflow eliminates PCR amplification, preventing error accumulation and delivering higher data accuracy. Ideal for researchers seeking reliable, high-fidelity WGS, the kit ensures robust performance across diverse genomic applications.

Product

Product Name	Item Number	Component	Component Item Number
MGIEasy PCR-Free DNA Library Prep Set (16 RXN)	1000013452	Library and Circularization: MGIEasy PCR-Free DNA Library Prep Kit	1000013456
		Adapter: MGIEasy PF Adapters-16 (tube) Kit	1000013460
		Beads: MGIEasy DNA Clean Beads (8 mL)	940-001596-00
MGIEasy PCR-Free DNA Library Prep Set (96 RXN)	1000013453	Core library: MGIEasy PCR-Free DNA Library Prep Kit	1000013457
		Adapter: MGIEasy PF Adapters-96 (plate) Kit	1000013461
		Beads: MGIEasy DNA Clean Beads (50 mL)	940-001594-00

Product Specification

Product Name	MGIEasy PCR-Free DNA Library Prep Set
Application	Whole genome sequencing
Version	V1.1
Configuration	16 RXN (1000013452) and 96 RXN (1000013453)
Validity period	9 months
Assay Time	~3.5 hrs
Input Quantity	80-200 ng fragmented DNA
Sample types	gDNA
Species Compatibility	Human, animals, plants, fungi, bacteria, metagenomics, etc.
Fragmentation Method	Sonication
Recommended Insert Size	350-400 bp
Platform Compatibility	DNBSEQ-G400, DNBSEQ-G50, DNBSEQ-T7
Recommended Read Length	SE100, PE100, PE150, PE200, SE400

Recommended Workflow

Application	Library Preparation	Sequencing	Analysis
Human WGS sequencing (High depth)	MGIEasy PCR-Free DNA Library Prep Set MGISP-100 MGISP-960	DNBSEQ-G400* DNBSEQ-G50* DNBSEQ-T7*	MegaBOLT Bioinformatics analysis accelerator WGS germline mutation analysis software WGS somatic mutation analysis software

Highlights

Compatible with Multiple Species

Supports DNA from humans, animals, plants, bacteria, fungi, including blood, saliva, fresh tissue, mice, rice, E. coli, and metagenomic samples.

Fast, Simple, Automated Workflow

Complete library preparation in 3.5 hours with size-selected DNA. The workflow is simple, automation-friendly, and avoids amplicon contamination.

No Amplification Error Accumulation

By eliminating PCR amplification, the PCR-free workflow on MGI DNBSEQ™ platforms prevents error accumulation, delivering high-fidelity genome data.

Higher Coverage Uniformity & Variant Detection

Reduces GC bias and improves coverage across the genome, including GC-rich, promoter, and repetitive regions, while enhancing sensitivity and accuracy for variant detection, especially indels.