MGIEasy rRNA Depletion Kit V1.3

The MGIEasy rRNA Depletion Kit V1.3 efficiently removes rRNA from human, mouse, and rat total RNA (10 ng–1 μg), leaving mRNA and other noncoding RNAs ready for downstream analysis. This kit works with both intact and degraded RNA, including FFPE samples, making it versatile for a wide range of research applications. When combined with the MGIEasy Fast RNA Library Prep Set, the resulting libraries are ideal for transcriptome profiling and noncoding RNA studies, delivering high-quality, reproducible results.

Designed for ease of use and broad RNA compatibility, this kit provides a reliable solution for researchers seeking accurate RNA analysis from challenging or low-input samples.

Product

Product Name Item Number Reactions
MGIEasy rRNA Depletion Kit V1.3 940-001751-00 16 RXN
MGIEasy rRNA Depletion Kit V1.3 940-001752-00 96 RXN

Product Specification

FunctionDeplete rRNA from human, mouse and rat total RNA (including cytoplasmic 5 S rRNA, 5.8 S rRNA, 18 S rRNA, 28 S rRNA, mitochondrial ribosomal RNA 12 S rRNA, 16 S rRNA and 45 S pre-ribosomal RNA)
Technical principlesRNase H digestion
ApplicationsWhole-Transcriptome Sequencing, Long non-coding RNA Sequencing
Species CompatibilityHuman, Mouse, Rat
Sample typesTotal RNA from blood, fresh tissue, FFPE RNA
Input quantity10 ng~1 μg total RNA
Assay time~ 2 hours
Adaptation automation platformMGISP-100 (16 RXN), MGISP-960 (96 RXN)

Recommended Workflow

ApplicationsRNA sample processingLibrary preparationSequencing
  • Whole-Transcriptome Sequencing (Human)
  • MGISP-100 / MGISP-960
  • RNA enrichment: MGIEasy rRNA Depletion Kit V1.3
  • MGISP-100 / MGISP-960
  • Library: MGIEasy Fast RNA Library Prep Set
  • MGIEasy Dual Barcode Circularization Kit (1000020570)
  • DNBSEQ-G400
  • DNBSEQ-G99
  • MGISEQ-200
  • DNBSEQ-T7
  • DNBSEQ-T10x4

Highlights

Efficient rRNA Removal

  • Effectively depletes rRNA while preserving mRNA and noncoding RNA for high-quality downstream analysis.

Supports Degraded Samples

  • Compatible with intact and degraded RNA, including FFPE samples, accommodating a wide range of RNA quality.

Automatable Workflow

  • Seamlessly integrates with MGISP-100 and MGISP-960 systems for streamlined, high-throughput RNA library preparation.

Flexible Library Compatibility

  • Works with various RNA sequencing library prep kits, providing adaptable solutions for different research needs.

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