MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0

DNA methylation plays a key role in regulating gene expression. The MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0 prepares fragmented, size-selected DNA into libraries optimized for MGI high-throughput sequencing platforms.

With improved algorithms, the kit minimizes or eliminates the need for balanced libraries, ensuring efficient and accurate genome-wide methylation sequencing. It enables single-base resolution analysis, delivering high-quality methylation maps for advanced epigenetic research.

Product

MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0 (16 RXN)
Item No.: 940-001530-00

Component Kits	Product Name of Component Kit	Part No. of Component Kit	Quantity of Component Kit in a Set
Core library & Adapter	MGIEasy Whole Genome Methylation Sequencing Library Prep Module (16 RXN)	940-001529-00	1
Circularization	MGIEasy Circularization Module (16 RXN)	1000005260	1
Clean Beads	MGIEasy DNA Clean Beads (15mL beads)	940-001174-00	1

MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0 (96 RXN)
Item No.: 940-001527-00

Component Kits	Product Name of Component Kit	Part No. of Component Kit	Quantity of Component Kit in a Set
Core library	MGIEasy Whole Genome Methylation Sequencing Library Prep Module (96 RXN)	940-001528-00	1
Adapter	MGIEasy Methylation Adapter and Single Barcode Primer Module (96 RXN)	940-001531-00	1
Circularization	MGIEasy Circularization Module (96 RXN)	1000017573	1
Clean Beads	MGIEasy DNA Clean Beads (75mL beads)	940-001526-00	1

Product Specification


Application	Genome-wide methylation sequencing
Conversion method	Compatible with enzymatic conversion method to convert unmethylated C to U
DNA Fragmentation method	Covaris (no fragmentation for cfDNA)
Library construction	Double stranded DNA library construction method
Sample types	Human gDNA extracted from blood, fresh tissue and cells samples and FFPE samples, and cell free DNA from plasma samples, gDNA from common plant and animal (such as mouse, Arabidopsis, etc.)
Input Quantity	100 ng ~ 1000 ng gDNA 5 ng ~ 20 ng cfDNA
Insert size	Single side size selection: 350 bp ~ 480 bp Dual side size selection: 350 bp ~ 450 bp
Barcode	~96 single barcodes
Circularization method	Single strand DNA circularization method
Assay Time	~10.5 h (from gDNA to ssCir DNA)
Sequencing platform & Recommended Read Length	DNBSEQ-G400 (ECR 7.0 and later), PE100 / PE150 DNBSEQ-T7 (ECR 5.0 and later), for use with DNBSEQ-T7RS High-throughput Methylation Sequencing Set (T7 Methylation FCL PE150)

Recommended Workflow

Applications	Library preparation	Sequencing	Data analysis
Human Whole Genome Methylation Sequencing	MGIEasy Whole Genome Methylation Sequencing Library Prep Kit V3.0	DNBSEQ-G400 DNBSEQ-T7	MegaBOLT-MethyScope Software

Highlights

Wide Sample Compatibility

Works with multiple sample types, including human gDNA from blood, fresh tissue, cell samples, FFPE samples, cell-free DNA from plasma, and gDNA from common plants and animals.

Streamlined Sequencing

Paired with the upgraded MGI sequencing algorithms, this kit eliminates the need for balanced libraries—simplifying workflows and improving data yield.