Besides genetic sequencers, library preparation products are crucial for advancing sequencing technology. MGI has developed a range of library preparation kits compatible with its own sequencing platforms.
The MGIEasy RNA Library Prep Set is part of MGI’s RNA library series, providing an efficient solution for generating libraries from 10 ng to 1 µg of total RNA. It is fully compatible with MGI high-throughput sequencing platforms.
The kit is user-friendly and widely applicable to transcriptome research in humans, animals, plants, and microbes. It enables comprehensive and rapid analysis of gene expression, rare and novel transcripts, alternative splicing events, gene fusions, SNPs, and allele-specific expression.
Data Performance
Libraries were prepared from Universal Human Reference RNA (UHRR) inputs ranging from 10 ng to 1 µg using the MGIEasy RNA Library Prep Set and sequenced on the MGISEQ-2000 at PE100 read length.
Across all RNA input amounts (1 µg, 200 ng, 50 ng, 10 ng), the kit delivers high-quality sequencing data with excellent genome and gene mapping rates, robust transcript detection, and consistent gene expression concordance.
Uniformity of coverage across the entire transcript is important for analysis of gene structure. A comparison was performed between the MGIEasy RNA Library Prep Set sequenced on an MGISEQ-2000 and other comparable RNA kits sequenced on another platform.
The results demonstrate that the libraries constructed using MGIEasy RNA Library Prep Set have superior 3’end coverage.
