MGISEQ-2000 Timeline & PE150 Sequencing Overview
MGISEQ-2000 Timeline
- 27 October 2017: ICG-12 debuts
- 14 February 2018: Delivered to BGI Genomics, Gene+, WeGene, RIKEN Japan and others
- Summer 2018: PE150 support introduced for MGISEQ-2000
About PE150 Sequencing
The PE150 reagent for MGISEQ-2000 provides high-throughput, accurate sequencing with consistent performance:
- Average run time: 3 days
- Effective reads per flow cell: up to 1,500M
- Lanes per flow cell: 4
- Effective reads per lane: ≥350M
- Maximal sequencing cycles: 320
Data Performance
PE150 has been validated across multiple experiments, showing stable performance with E. coli samples:
- Total reads: ≥350M per lane
- Q30 quality score: consistently above 85%
Reproducibility
| Test | Total Reads CV | Q30 CV |
|---|---|---|
| Same batch of flowcells & reagents, 6 lanes | 3.24% | 1.13% |
| 3 reagent batches, 25 lanes | 3.34% | 0.93% |
| 3 instruments, 20 lanes | 2.76% | 0.93% |
| 3 batches of flowcells, 6 lanes | 4.96% | 1.79% |
Applications
PE150 demonstrates reliable performance across a wide variety of libraries:
- Whole genome sequencing (WGS) – including PCR-free
- Transcriptome analysis
- Region capture & amplicon sequencing
- Tumour panel sequencing
WGS Performance
- Sample: NA12878
- Base quality: Q20 97.2%, Q30 91.3%
- SNP detection sensitivity: 99.48%
- Indel detection sensitivity: 95.03%
Transcriptome Performance
- Sample: Universal Human Reference RNA (UHRR)
- MGISEQ-2000 showed ~2% higher genome mapping ratio and ~6% higher gene mapping ratio compared to N sequencing platform using B library prep kit
- Gene and transcript numbers comparable across platforms
- Coverage uniformity along gene bodies (5′–3′) comparable or better than other platforms
Summary
PE150 for MGISEQ-2000 ensures high-quality, reproducible sequencing for a wide range of applications. With stable performance, high sensitivity, and excellent coverage, PE150 is now available for order.
Total reads CV=3.24%, data quality Q30 CV=1.13%
Reproducibility between 3 reagent batches (analyzing 25 lanes)
Total reads CV=3.34%, data quality Q30 CV=0.93%
Reproducibility between 3 instruments (analyzing 20 lanes)
Total reads CV=2.76%, data quality Q30 CV=0.93%
Reproducibility between 3 batch of flowcells (analyzing 6 lanes)
Total reads CV=4.96%, data quality Q30 CV=1.79%
A wide range of applications
Stable and excellent performance with a variety of libraries tested: whole genome (including PCR-free), transcriptome, region capture, amplicon, tumor panel and more
- WGS
- Sample source: NA12878
- Distribution of MGISEQ-2000 reads base and quality
Summary of WGS performancebetween sequencing platforms
Transcriptome
Sample source: UHRR
The Genome mapping ratio on the MGISEQ-2000 platform is about 2% higher than the N sequencing platform, and the Gene mapping ratio is about 6% higher than the B library prep kiton the N sequencing platform.
The MGISEQ-2000 platform showed similar results as of the N sequencing platform on Gene number and Transcript number.
Random comparison of reads between sequencing platforms
The randomness of the MGISEQ-2000 platform and the N sequencing platform of the B library prep kitis comparable, and the coverage at the 3′ end is better than that of the A library prep kiton the N sequencing platform.
● Of course, the advantages of PE150 reagent for MGISEQ-2000RS high-throughput genetic sequencer are far more than the above.
● Now PE150 is here waiting for you
