MGISEQ-2000RS FCS Sequencing Reagent Kit Overview
The MGISEQ-2000RS FCL sequencing kit offers high throughput and low cost per Gb. However, in scenarios with limited sample numbers, users may not fully utilise all lanes—resulting in increased per-run costs.
To address this, the MGISEQ-2000RS FCS Sequencing Reagent Kit was developed. It provides:
Lower output per run for better lane utilisation
Increased flexibility for varying sample sizes
Faster sequencing speeds
Optimised cost efficiency
This enables users to run more experiments, faster and more economically.
Key Applications Tested
Early-access users validated the FCS kit across a wide range of sample and library types, including:
- Tissue samples
- FFPE
- cfDNA / ctDNA
- gDNA
- Multiplex PCR
- Hybridisation products
- Tumour standards (various mutation frequencies)
Results showed high data quality and output exceeding expectations.
User Feedback
Research Science: Smaller data volume allows more flexible runs and shorter experiment time.
CWBIO: PE150 is ideal for mutation detection requiring longer reads, while maintaining delivery time and high data quality.
JingKe Biotech: Suitable for small and mid-sized labs with cost-effective and flexible sequencing.
Gene+: Data quality and output meet expectations; looking forward to full release.
Key Features of FCS Reagent Kit
1. Faster Sequencing Speed
- ~50% faster than FCL at the same read length
- PE100: ~26 hours
- PE150: ~37 hours
2. No Wasted Lanes
- ~550M reads per slide
- Easier lane saturation with fewer samples
- Reduced operational costs
3. High Data Quality
- Comparable quality to FCL
- Maintains high Q30 and accuracy metrics
4. Cross-Platform Compatibility
- Compatible with MGISEQ-2000RS
- Supports upcoming MGISEQ-2000RS FAST platform
Performance Results
PE150 Performance
- Total Reads: >300M per lane
- Q30: >88%
- Split Rate: >98%
- Low variability (CV < 2.5%)
PE100 Performance
- Total Reads: >300M per lane
- Q30: >88%
- Split Rate: >98%
- Stable and consistent performance
Tumour Detection Performance
- High-frequency variants (>3%): ~99–100% detection rate
- Mid-frequency (1–3%): Within expected range
- Low-frequency (<1%): Stable and comparable to FCL
FCS showed slightly better performance in high-frequency mutation detection.
Comprehensive Evaluation
After extensive testing, the FCS reagent kit demonstrates:
- High data accuracy
- Strong mutation detection capability
- Excellent stability
- Greater flexibility and cost efficiency
It is an ideal solution for labs needing mid-throughput sequencing with optimised resource usage.
Product Information
Product Name | Version | Item Number |
|---|---|---|
MGISEQ-2000RS High-throughput Rapid Sequencing Set (FCS PE100) | V1.0 | 1000013155 |
MGISEQ-2000RS High-throughput Rapid Sequencing Set (FCS PE150) | V1.0 | 1000011718 |
