ATOPlex Fast NiV Whole Genome Targeted Sequencing Package
The ATOPlex Fast NiV Whole-Genome Targeted Sequencing Package enables rapid, sensitive Nipah virus whole-genome sequencing on DNBSEQ or CycloneSEQ platforms. Designed for RNA extracted from clinical and environmental samples, the workflow combines streamlined library preparation, high detection sensitivity, and flexible short- and long-read sequencing — delivering accurate genomic analysis with faster turnaround.
Download the flyer for full NiV workflow details, platform specifications and ordering information.
Other Material
Product Components
Short-read sequencing solution（DNBSEQ）
| Category | Product Name | Part Number |
|---|---|---|
| Genetic Sequencers | DNBSEQ-E25RS Genetic Sequencer | 900-000537-00 |
| DNBSEQ-G99RS Genetic Sequencer | 900-000607-00 | |
| DNBSEQ-G99ARS Genetic Sequencer | 900-000609-00 | |
| Automation Instruments | Automated Nucleic Acid Extractor MGISP-NE32RS | 950-000020-00 |
| DNA Sequencing Library Preparation System MGISP-100RS | 900-000206-00 | |
| Nucleic acid extraction reagents | MGIEasy Nucleic Acid Extraction Kit OP02-32 (32 RXN) | 1000023774 |
| MGIEasy Nucleic Acid Extraction Kit T-96 (96RXN) | 1000020471 | |
| MGIEasy Microbiome DNA&RNA Extraction Kit PMT-48 48Preps/Kit (48RXN) | 940-002223-00 | |
| MGIEasy Microbiome DNA&RNA Extraction Kit (96RXN) | 940-002222-00 | |
| Library preparation reagents | ATOPlex Fast NiV Library Prep Set (for DNBSEQ) (16 RXN) | 940-003133-00 |
| ATOPlex Fast NiV Library Prep Set (for DNBSEQ) (96 RXN) | 940-003132-00 | |
| Sequencing reagents | DNBSEQ OneStep DNB Make Reagent Kit V4.0 (OS-Dual Barcode) (4 RXN) | 940-001654-00 |
| DNBSEQ-G99 High-throughput Sequencing Set (G99 FCL PE150) | 940-001269-00 | |
| DNBSEQ-E25RS High-throughput Sequencing Set (FCL PE150) | 940-000567-00 | |
| Data analysis | ATOPlex MultiPCR Microbiome Research Software Analysis Package (16 reports) | 970-000528-00 |
| ATOPlex MultiPCR Microbiome Research Software (ATOPlex MMRS) | 970-000518-00 | |
| OmicsNest Bioinformatics Platform | 900-001195-00 |
Long-read sequencing solution（CycloneSEQ）
| Category | Product Name | Part Number |
|---|---|---|
| Genetic Sequencers | Nanopore Genetic Sequencer G100-ER | H900-000048 |
| Nanopore Genetic Sequencer Bundle 2( G100-ER sequencer+work-station+Keyboard & Mouse+Display Screen) | H910-000022 | |
| Automation Instruments | Automated Nucleic Acid Extractor MGISP-NE32RS | 950-000020-00 |
| DNA sequencing Library Preparation System MGISP-100RS | 900-000206-00 | |
| Nucleic acid extraction reagents | MGIEasy Nucleic Acid Extraction Kit OP02-32 (32 RXN) | 1000023774 |
| MGIEasy Nucleic Acid Extraction Kit T-96 (96RXN) | 1000020471 | |
| MGIEasy Microbiome DNA&RNA Extraction Kit PMT-48 48Preps/Kit (48RXN) | 940-002223-00 | |
| MGIEasy Microbiome DNA&RNA Extraction Kit (96RXN) | 940-002222-00 | |
| Library preparation reagents | ATOPlex Fast NiV Library Prep Set (for CycloneSEQ) (24RXN) | 940-003130-00 |
| ATOPlex Fast NiV Library Prep Set (for CycloneSEQ) (96RXN) | 940-003131-00 | |
| Sequencing reagents | WT Sequencing Flow Cell (2 pcs/set) | H930-000002-00 |
| CycloneSEQ WT Sequencing Kit (6 T) | H940-000016 | |
| Data analysis | ATOPlex MultiPCR Microbiome Research Software Analysis Package (16 reports) | 970-000528-00 |
| ATOPlex MultiPCR Microbiome Research Software (ATOPlex MMRS) | 970-000518-00 | |
| OmicsNest Bioinformatics Platform | 900-001195-00 |
Product Parameters
| Parameter | DNBSEQ solution | CycloneSEQ solution |
|---|---|---|
| Sample type | RNA extracted from serum, cerebrospinal fluid, tissue, swabs, or the environment, etc. | RNA extracted from serum, cerebrospinal fluid, tissue, swabs, or the environment, etc. |
| Amplicon region | Nipah virus whole genome (Malaysian strain (NiV-M) and Bangladeshi strain (NiV-B)) | Nipah virus whole genome (Malaysian strain (NiV-M) and Bangladeshi strain (NiV-B)) |
| Amplicon length | 200 ~ 300 bp | 200 ~ 300 bp or 600 ~ 800 bp are available |
| Amplicon count | 90 pairs | 90 or 30 pairs are available |
| Number of amplification tubes | 1 tube | 1 tube |
| Recommended RNA input | 10 μL | 10 μL |
| Automation | SP-100 | SP-100 |
| Library-prep time | 4.2 h (Including make DNB) | 3.5 h |
| Recommended sequencing platforms | E25 , G99 | G100-ER |
| Sequencing read | PE150+10+10 | / |
| Recommended sample size | Ct ≤ 28 : E25(8 samples/FC) ; G99 (32 samples/FC)Ct > 28 /Unknown Ct value : E25(4 samples/FC) ; G99 (16 samples/FC) | Ct ≤ 28 : G100-ER (8 samples/FC)Ct > 28 /Unknown Ct value : G100-ER (4 samples/FC) |
| Bioinformatics analysis software | ATOPlex MMRS | ATOPlex MMRS |
| Recommended analysis data volume per sample | Ct ≤ 28 : 2.5M reads/sampleCt > 28 /Unknown Ct value : 5M reads/sample | Ct ≤ 28 : 1 Gb/sampleCt > 28 /Unknown Ct value : 2 Gb/sample |
| Variant types | SNV , INDEL | SNV , INDEL |
| Key advantages | Cost-effective | Real-time sequencing for faster turnaround |
Key Features
Convenient and Quick Library Preparation
NiV primer pools combined with ATOPlex Fast series kits simplify library preparation by eliminating end-repair and A-tailing. With 5-minute rapid adapter ligation and just four steps from RNA to library, library construction can be completed in 3.5 hours and is compatible with MGI automated systems.
High Detection Sensitivity
No sample culture is required. The workflow directly detects RNA from human serum, cerebrospinal fluid, tissue, swab, environmental and food samples, enabling high-coverage whole-genome sequencing even from samples with low viral loads.
Supports Long- and Short-Read Sequencing
Compatible with both CycloneSEQ and DNBSEQ platforms, the solution combines cost-effective short-read sequencing with real-time long-read sequencing. Samples can be processed immediately without batching, helping shorten turnaround times across diverse applications.
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