Dengue Virus Sequencing Package
MGI’s DENV1–4 Sequencing Package offers a fast, full-process solution for accurate detection and typing of all dengue virus serotypes. Built on ATOPlex multiplex PCR technology, it enables sensitive amplification from low-copy plasma or serum samples and supports both DNBSEQ and CycloneSEQ platforms. The workflow includes nucleic acid extraction, one-tube library prep, high-throughput sequencing, and rapid data analysis—with results available in as little as 12 hours (DNBSEQ). Ideal for outbreak response and surveillance, it delivers full-genome coverage and reliable variant detection in a simple, cost-effective package.
DNBSEQ Solution Components & Part Numbers
| Part Number | |
|---|---|
| DNBSEQ Solution | |
| Genetic Sequencer G99ARS | 900-000609-00 |
| SP-NE32RS Automated Nucleic Acid Extractor | 950-000020-00 |
| DNA Sequencing Library Preparation System MGISP-100RS | 900-000206-00 |
| ATOPlex DENV1-4 Library Preparation Set (96 RXN) | 940-002196-00 |
| G99RS High-throughput Sequencing Set (G99 FCL SE100/PE50) | 940-001268-00 |
| DNBSEQ OneStep DNB Make Reagent Kit (OS-DB) (4 RXN) | 1000026466 |
| MGI metargetCOVID software | 970-000228-00 |
CycloneSEQ Solution Components & Part Numbers
| Part Number | |
|---|---|
| CycloneSEQ Solution | |
| Nanopore Gene Sequencer Bundle 2 (CycloneSEQ-WT02 sequencer + workstation + keyboard & mouse + display screen) | H900-000003-00 |
| DNA Sequencing Library Preparation System SP-100RS | 900-000206-00 |
| MGIEasy Nucleic Acid Extraction Kit (96 RXN) | 1000020471 |
| ATOPlex RNA Multiplex PCR Amplification Set V3.1 (96 RXN) | 940-000135-00 |
| MGIEasy DNA Fast Library Prep Set (for CycloneSEQ) (24 RXN) | 940-002654-00 |
| WT Sequencing Flow Cell (2 pcs/set) | H930-000002-00 |
| CycloneSEQ WT Sequencing Kit (6 T) | H940-000016 |
| ATOPlex MultiPCR Microbiome Research Software (ATOPlex MMRS) | 970-000518-00 |
Dengue Virus Sequencing Package Components & Part Numbers
| Part Number | |
|---|---|
| Dengue Virus Sequencing Package | |
| ATOPlex DENV1-4 Amplification Set (16 RXN) | 940-002702-00 |
| MGIEasy DNA Fast Library Prep Set (for CycloneSEQ) (24 RXN) | 940-002654-00 |
| ATOPlex MultiPCR Microbiome Research Software (ATOPlex MMRS) | 970-000518-00 |
Sequencing Package Technical Specs
| Parameter | DNBSEQ Solution | CycloneSEQ Solution |
|---|---|---|
| Sequencing Package Technical Specs | ||
| Sample Type | Plasma, serum, and other samples | Plasma, serum, and other samples |
| Amplification Area | Full-length genomes of DENV1-4 | Full-length genomes of DENV1-4 |
| Amplicon Size | 145–165 bp | 145–165 bp |
| Amplicon Number | 436 | 436 |
| Number of Amplification Tubes | 1 tube | 1 tube |
| Recommended RNA Input Amount | 10 µL, >100 copies/RXN | 10 µL, >100 copies/RXN |
| Automated Library Preparation | SP-100/D4 | SP-100/CyclonePrep-4 |
| Library Preparation Time | 6 hours | 3.5 hours |
| Sequencing Platform | E25, G99/G99ARS, G50 | G100-ER |
| Read Length | SE100/PE100 | N/A |
| Recommended Data Volume | SE100: 5M reads (Ct ≤30); 10M reads (Ct >30) | 500M bases |
| Detection Type | SNV, INDEL | SNV, INDEL |
| Solution Advantages | Cost-effective | Real-time sequencing; faster turnaround |
Key Features
Full DENV1–4 Coverage
Amplifies the complete genomes of all four dengue virus serotypes with high accuracy.
Rapid Results
Get sequencing and analysis results in as little as 12 hours using DNBSEQ.
Easy, One-Tube Workflow
Simplified ATOPlex library prep with minimal hands-on time and low input volume.
High Sensitivity
Detects low viral loads, ideal for early-stage infections and clinical screening.
Multiple Sample Types
Compatible with plasma, serum, and other sample types for flexible testing.
Dual Platform Support
Works with both DNBSEQ and CycloneSEQ platforms to fit different lab setups.
Discover More
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📞(+86) 4000-688-114
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