Environmental DNA Metabarcoding Sequencing Package

The MGI Environmental DNA Metabarcoding Sequencing Package is a complete, end-to-end solution for environmental monitoring — from sample collection to insightful reporting. It integrates self-developed reagents for nucleic acid extraction, ATOPlex multiplex PCR library preparation, an eDNA sampler, the SP-960 automated sample prep system, and powerful MetaSIS analysis software.

At the heart of the workflow are MGI’s cutting-edge G99 and E25 sequencers, delivering rapid and accurate analysis of biological community structures within environmental samples. This package is designed to streamline ecological research and biodiversity monitoring with efficiency, precision and ease.

Components

MGI DNBSEQ-G99 ultra-fast benchtop genetic sequencer for rapid genomic workflows and mid-throughput sequencing.
MegaBOLT
E25

One-stop solution

Complete workflow: sampling, treatment, library prep, sequencing, and analysis.

Wide species coverage

Monitors bacteria, fungi, algae, plants, and animals across ecosystems.

Automation friendly

Supports automated extraction, prep, and data analysis to save time.

Powerful analysis

Advanced software for OTU, diversity, composition, and biomarker analysis.

Performance Parameters

PanelApplicable species typesRecommended read lengthRecommended data per sampleSequencerRecommended sample number /FC
16S V4bacteriaPE150≥65K readsG99 / E25500/72
MiFishfishPE150≥65K readsG99 / E25500/72
16S V3V4bacteriaPE300≥65K readsG99300
COIbenthic animals, zooplanktonPE300≥65K readsG99300
Ac12SfishPE300≥65K readsG99300
18S V4algae, invertebratesPE300≥65K readsG99300
ITS1FungusPE300≥65K readsG99300
ITS2FungusPE300≥65K readsG99300

Workflow

Environmental DNA Metabarcoding Sequencing Package

Publication list

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