Fluorometer 1
The Fluorometer 1 is a fluorescence-based quantitative device that delivers accurate measurements of DNA, RNA and protein concentrations. Designed for single-sample detection, it requires only 1–20 μL of sample to generate precise concentration readings, offering high sensitivity while minimising sample consumption. Fast, reliable and easy to use, the instrument is ideal for applications including DNA quantification for NGS library construction, RNA quantification and nucleic acid concentration analysis in PCR experiments.
Equipped with a high-sensitivity silicon photomultiplier tube detector (SiPMT), the Fluorometer 1 provides exceptional sensitivity for trace amounts of dsDNA, ssDNA, RNA and proteins without cross-interference. The system supports virtually any fluorescent assay, eliminating the need for proprietary kits. Compact (110 × 230 × 45.5 mm) and lightweight (0.5 kg), it saves valuable bench space while remaining highly portable for everyday laboratory use.
Other Material
Product Overview
Performance Parameters
| Description / Product Specification | Single-channel fluorometer | ||
| Sample volume required | 1–20 μL | ||
| Sample throughput | 1 | ||
| Compatible consumables | 0.5 mL clear PCR tube | ||
| Detection time | ≤5 seconds per sample | ||
| Linear range | 5 orders of magnitude | ||
| Light source | High-efficiency monochromatic LED | ||
| Detection channels | 2 | ||
| Excitation wavelength | Blue 460–480 nm, Red 630–650 nm | ||
| Emission wavelength | Green 500–535 nm, Red 670–710 nm | ||
| Detection device | SiPMT | ||
| Sensitivity | dsDNA 0.01 ng/μL | ||
| Assay types | dsDNA High Sensitivity, dsDNA Broad Range, RNA High Sensitivity, RNA Broad Range, RNA Extended Range, microRNA, ssDNA, Protein Broad Range, Kinetic | ||
| Calibration curve | 2-point calibration | ||
Applications
NGS Workflows
Primarily used for whole-genome sequencing and NGS library preparation, enabling accurate quantification and quality assessment of sequencing libraries to ensure high-quality downstream results.
Nucleic Acid Amplification
Suitable for the quantification of end-point PCR products and cDNA after RT-PCR. It also provides precise template quantification required before real-time quantitative PCR (qPCR).
Cell & Genetic Engineering
Used for transfection efficiency assessments, such as marker gene detection. Ideal for DNA/RNA quantification following gene editing (e.g., CRISPR) and quality control prior to transfection.
Protein Analysis
Supports sample quantification before protein gel electrophoresis and assessments of labeling efficiency for Western blots. Effective for determining enzyme activity in various research workflows.
Order Information
| Item NO. | Product Name | Product Description | Catalog # |
| 950-000238-00 | Fluorometer | Single-channel fluorometer | Fluorometer 1 |
Recommended Reagents
| Item NO. | Product Name | Product Specifications | |
| 961-000718-00 | ssDNA Assay kit | 500T | |
| 961-000719-00 | ssDNA Assay kit | 100T | |
| 961-000720-00 | RNA HS Assay kit | 500T | |
| 961-000721-00 | 1x dsDNA HS Assay kit | 500T | |
| 961-000722-00 | dsDNA HS Assay kit | 500T | |
| 961-000723-00 | dsDNA BR Assay Kit | 500T | |
| 961-000724-00 | 1x dsDNA HS Assay Kit | 100T | |
| 961-000725-00 | dsDNA HS Assay Kit | 100T | |
Recommended Consumables
| Item NO. | Product Name | Product Specifications | |
| 962-000466-00 | Qubit Assay Tubes | 500 tubes/box | |
| 962-000467-00 | Qubit Assay Tubes | 10 box per ton | |
Features
High-Sensitivity Detection
- Advanced SiPMT detector
- Precise capture of weak fluorescence signals
- Accurate and reliable quantification
Rapid Micro-Volume Analysis
- Results within 3–5 seconds
- Requires only 1–20 μL sample
- Fast and efficient detection
Accurate Molecular Identification
- Detects dsDNA, ssDNA, RNA and proteins
- High specificity and sensitivity
Flexible Reagent Compatibility
- Compatible with various fluorescent reagents
- No proprietary kit restrictions
Power, Dimensions & Net Weight
- Voltage: DC5V, 2A
- Rated Power: 10W
- Dimensions (W×D×H): 230 mm × 110 mm × 45.5 mm
- Net Weight: 0.5Kg
Operating Environment Requirements
- Temperature: 15°C – 30°C
- Relative Humidity: ≤75% RH
- Indoor use only.
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