HIV-1 Full-Length Genome Sequencing Package

HIV-1’s high mutation, replication, and recombination rates drive extreme genetic diversity and rapid evolution, complicating disease control, accelerating drug resistance, and challenging clinical treatment. Limited cross-immune responses between subtypes also hinder vaccine development. MGI’s HIV-1 Full-Length Genome Sequencing Package addresses these challenges with a complete workflow from viral RNA to report.

Based on CycloneSEQ nanopore technology and paired with the ATOPlex library kit and automated sample handling, it enables precise subtyping, superinfection analysis, quasispecies identification, and structural variation detection, covering the entire nearly 9.8 kb genome without assembly errors for accurate research and clinical insights.

Other Material

[PDF Brochure] HIV-1 Full-Length Genome Sequencing Package

Product Components & Part Numbers

Category	Product	Cat. NO.
Instruments	G100-ER Nanopore Gene Sequencer	H900-000048
	G100-ER Nanopore Genetic Sequencer Bundle 2	H910-000022
	SP-100RS DNA sequencing Library Preparation System	900-000206-00
Library prep reagents	ATOPlex HIV-1 FL Amplification Set	940-002907-00
	MGIEasy DNA Fast Library Prep Set (for CycloneSEQ)	940-002966-00
Sequencing reagents	WT Sequencing Flow Cell	H930-000002-00
	CycloneSEQ WT Sequencing Kit	H940-000016
	CycloneSEQ WT Flow Cell Wash Kit	H940-000098
Data analysis	HIVLongSeq	970-000557-00
	CycloneSEQ Workstation	—
	HIV-GenomePro Platform	900-000818-00
	OmicsNest Bioinformatics Platform	900-001195-00
	Platform of microorganisms Fast Identification	900-000393-00

Product Specification

Parameter	Specification
Intended Use	Accurate genotyping, superinfection analysis, quasispecies identification, and drug resistance analysis of HIV-1
Targeted Region	HIV-1 near-full-length genome (~9000 bp)
Sample Types	Viral RNA extracted from plasma or cultured strains
Compatible Platform	CycloneSEQ G100-ER Sequencer
Recommended Data Volume	>135K raw reads/sample
Sample Requirements	Ct≤30 or ≥5000 cps/rxn
Recommended Throughput	≤24 samples/FC

System Parameters

Unraveling HIV-1 Genetic Diversity

CycloneSEQ nanopore sequencing resolves complete viral genomes without PCR, preserving quasispecies diversity and avoiding amplification bias.

Automation-Friendly

Paired with MGISP-100 and analysis software, it automates library prep and data analysis, reducing manual work.

Simple Library Preparation

Reverse transcription of 5' and 3' ends occurs in a single reaction tube, boosting stability and efficiency.

Comprehensive Analysis Capabilities

Full-length genome assembly, precise typing, superinfection and quasispecies analysis, drug resistance annotation, and hybrid assembly from CycloneSEQ and DNBSEQ.