Monkeypox Virus Sequencing Package
As Monkeypox (MPXV) cases rise globally, rapid and precise detection is critical for effective outbreak control. Powered by DNBSEQ™ and CycloneSEQ technologies, this integrated package delivers near-complete genome coverage and high-depth accuracy for real-time mutation tracking and epidemiological insights.
By leveraging patented ATOPlex technology for targeted detection, labs can achieve precise genome assembly and evolutionary tracing. For unbiased surveillance, the PFI (Platform for Fast Identification) enables the detection of MPXV variants and co-circulating pathogens, while dedicated MPXV Software automates the transition from library prep to actionable results. This streamlined workflow equips labs with the technical precision required for a decisive global health response.
Other Material
Product Components & Part Numbers
| Part Number | |
|---|---|
| DNBSEQ Targeted Sequencing Solution | |
| Genetic Sequencer G99 / E25 / G50 / G400 | – |
| DNA Sequencing Library Preparation System SP-100RS | 900-000206-00 |
| High-throughput Automated Sample Preparation System SP-960RS Config7 | 900-000152-00 |
| MGIEasy Microbiome DNA Extraction Kit MD01T-96（96 RXN） | 1000027955 |
| ATOPlex MPXV Amplification Set（96 RXN） | 940-002700-00 |
| ATOPlex MultiPCR Microbiome Research Software（ATOPlex MMRS） | 970-000518-00 |
| MGI MPXV Assembly and Phylogenetic Software Analysis Package | – |
| CycloneSEQ Targeted Sequencing Solution | |
| Nanopore Gene Sequencer Bundle 2 (CycloneSEQ-WT02 + accessories) | H900-000003-00 |
| DNA Sequencing Library Preparation System SP-100RS | 900-000206-00 |
| High-throughput Automated Sample Preparation System SP-960RS Config7 | 900-000152-00 |
| MGIEasy Microbiome DNA Extraction Kit MD01T-96（96 RXN） | 1000027955 |
| ATOPlex MPXV Amplification Set（96 RXN） | 940-002700-00 |
| MGIEasy DNA Fast Library Prep Set (for CycloneSEQ) (24 RXN) | 940-002654-00 |
| WT Sequencing Flow Cell (2 pcs/set) | H930-000002-00 |
| CycloneSEQ WT Sequencing Kit (6 T) | H940-000016 |
| ATOPlex MultiPCR Microbiome Research Software（ATOPlex MMRS） | 970-000518-00 |
Workflow
Performance Summary
| Parameter | DNBSEQ Targeted | CycloneSEQ Targeted | DNBSEQ Metagenomic |
|---|---|---|---|
| Genome Target | Monkeypox virus whole genome (NC_063383.1) | Monkeypox virus whole genome (NC_063383.1) | Non-targeted (metagenomic approach) |
| Amplicon Length | 313–395 bp | 313–395 bp | N/A |
| Library Preparation Time | ≤9 hours | ≤4.5 hours | ≤4 hours |
| Genome Coverage | Up to 99% | Up to 99% | Varies by read depth |
| Recommended Platforms | G99 / E25 / G50 / G400 | CycloneSEQ-WT02 | G99 / G50 / G500 |
| Read Length | SE100+10+10 or PE150+10+10 | N/A (real-time nanopore) | PE100 |
| Analysis Software | MPXV Assembly + ATOPlex MMRS | ATOPlex MMRS | MPXV Assembly & Phylogenetic Software |
| Mutation Detection | SNVs, INDELs | SNVs, INDELs | SNVs, INDELs |
| Solution Strength | Cost-effective | Real-time, fast turnaround | Ideal for mixed/unknown pathogens |
Our Monkeypox sequencing solutions are designed to work with a variety of clinical samples for comprehensive detection and analysis. These include herpes fluid, throat swabs, and nasal swabs, all fully compatible with DNBSEQ, CycloneSEQ, and metagenomic sequencing workflows. Additionally, other swab samples such as lesion swabs can be tested effectively, provided the viral load is sufficient—typically with a Ct value of 32 or lower recommended for reliable results.
Key Features
End-to-End Solution
From sample extraction to variant analysis, everything you need is in one streamlined workflow.
High Sample Throughput
Automated platforms like MGISP-960 and MGISP-100 enable large-scale processing with minimal manual work.
Dual Sequencing Platforms
Compatible with both short-read DNBSEQ and long-read CycloneSEQ technologies for flexible deployment.
Near-Complete Genome Coverage
Achieve up to 99% coverage of the Monkeypox virus genome for confident detection and surveillance.
Mutation & Traceability Analysis
Accurately detect SNVs and INDELs, perform traceability studies, and monitor pathogen evolution.
Rapid Library Preparation
Prepare libraries in as little as 4.5 hours with CycloneSEQ — fast turnaround without compromising quality.
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Get in Touch
Phone
📞(+86) 4000-688-114
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