Mycobacterium tuberculosis Sequencing Package
The Mycobacterium tuberculosis Sequencing Package provides a complete end-to-end workflow for TB detection, strain typing and drug resistance analysis. Combining MGI’s automated sample preparation, DNBSEQ short-read sequencing and CycloneSEQ long-read sequencing, the solution enables accurate genome analysis, rapid identification and reliable surveillance of Mycobacterium tuberculosis. Integrated bioinformatics tools support variant detection, resistance prediction and phylogenetic analysis, helping researchers and public health laboratories better understand TB transmission and evolution.
Product Parameters & Specs
| Parameter | Targeted Sequencing (ATOPlex) | WGS – DNBSEQ Solution | WGS – CycloneSEQ Solution |
|---|---|---|---|
| Primary Application | MTBC identification, strain typing, resistance mutation detection | Whole genome sequencing for MTB strain characterisation | Complete MTB genome assembly using long-read sequencing |
| Sample Type | Sputum, lavage fluid, cultured isolates | MTB cultured isolates | MTB cultured isolates |
| Recommended DNA Input | 1–10 ng (clinical samples) / ≥1 ng (culture) | ≥10 ng | ~500 ng |
| Library Preparation | ATOPlex multiplex PCR library preparation | MGIEasy Fast Enzymatic DNA Library Prep | MGIEasy Rapid DNA Library Prep (CycloneSEQ) |
| Sequencing Platform | DNBSEQ-E25, DNBSEQ-G99 | DNBSEQ-G99, MGISEQ-200 | CycloneSEQ G100-ER |
| Read Length | PE150 + indexing | PE150 | Long-read sequencing |
| Bioinformatics Software | MTB-Explorer | MTB-Explorer | MGA microbial genome assembler |
Performance & Data Output
| Metric | Targeted Sequencing | WGS – DNBSEQ | WGS – CycloneSEQ |
|---|---|---|---|
| Recommended Data Output | ~3M reads per sample | ~5M reads per sample | Genome depth ≥50× |
| Variant Detection | 40,000+ MTB variants covered | Genome-wide variant detection | Structural variation detection and complete genome assembly |
| Drug Resistance Analysis | 13,000+ resistance mutations | Comprehensive genomic resistance profiling | Long-read structural mutation detection |
| Species Identification | MTBC identification and spoligotyping | Strain-level MTB identification | High-resolution phylogenetic analysis |
| Drug Coverage | 15 first- and second-line TB drugs | Genome-wide resistance markers | Genome-scale resistance analysis |
Product Configuration
| Product | Part Number / Details |
|---|---|
| ATOPlex MTB Targeted Sequencing Suite | |
| DNBSEQ-G99ARS Gene Sequencer | 900-000608-00 |
| DNBSEQ-G99RS Gene Sequencer | 900-000611-00 |
| ATOPlex MTB Library Prep Kit (96 RXN) | 940-001601-00 |
| ATOPlex MTB Library Prep Kit (576 RXN) | 940-001599-00 |
| DNBSEQ One-Step DNB Prep Kit V4.0 (4 RXN) | 940-001654-00 |
| DNBSEQ-G99RS Reagent Kit (G99 FCL PE150) | 940-001269-00 |
| MTB-Explorer Software (16-person) | 970-000397-00 |
| MTB WGS Sequencing Suite (DNBSEQ) | |
| MGISP-100RS Automated Sample Prep (Standard) | 900-000206-00 |
| DNBSEQ-G99ARS Gene Sequencer (High-end) | 900-000609-00 |
| MGIDL-G99 | 510-003170-00 |
| MTB-Explorer Software | 970-000385-00 (Used together with the bioinformatics analysis server) |
| MGIEasy Fast FS DNA Library Prep Set V2.0 (16 RXN) | 940-001193-00 |
| MGIEasy Fast FS DNA Library Prep Set V2.0 (96 RXN) | 940-001194-00 |
| MGIEasy Fast UDB PF Adapter Kit (16 RXN) | 1000022800 |
| MGIEasy Fast UDB PF Adapter Kit (96 RXN) | 1000022802 |
| MGIEasy DNA Clean Beads (8 mL) | 1000005278 |
| MGIEasy DNA Clean Beads (50 mL) | 1000005279 |
| DNBSEQ OneStep DNB Make Reagent Kit V4.0 (4 RXN) | 940-001654-00 |
| DNBSEQ-G99RS High-throughput Sequencing Set (G99 FCL PE150) | 940-001269-00 |
| MTB WGS Sequencing Suite (CycloneSEQ) | |
| Nanopore Gene Sequencer Combo 2 (G100-ER + Workstation) | H910-000020 |
| MGISP-NE32RS Nucleic Acid Extraction System | 950-000019-00 |
| MGISP-100RS Gene Sequencing Lib Prep Instrument | 900-000070-00 |
| MGIEasy Microbial DNA & RNA Co-Extraction Kit (PMT-96) | 940-002222-00 |
| MGIEasy DNA Rapid Lib Prep Kit (CycloneSEQ, 24 RXN) | 940-002654-00 |
| WT Sequencing Chips (2/set) | H930-000001-00 |
| CycloneSEQ WT Sequencing Kit (6 tests/kit) | H940-000016 |
| Microbial Assembly Software (MGA) | 970-000527-00 |
| Rapid Microbial ID Platform (PFI) | 900-000392-00 |
Workflow & Compatibility
| Workflow Step | Targeted Sequencing | WGS – DNBSEQ | WGS – CycloneSEQ |
|---|---|---|---|
| Nucleic Acid Extraction | Direct extraction from sputum or lavage samples | MGIEasy Microbial DNA & RNA Extraction Kit | MGIEasy Microbial DNA & RNA Extraction Kit |
| Library Preparation | ATOPlex MTB Library Preparation Kit | MGIEasy Fast DNA Library Prep | MGIEasy Rapid DNA Library Prep |
| Sequencing | DNBSEQ-E25 / DNBSEQ-G99 | DNBSEQ-G99 / MGISEQ-200 | CycloneSEQ G100-ER |
| Data Analysis | MTB-Explorer | MTB-Explorer | MGA microbial genome analysis platform |
Key Features
Culture-Free Detection
- High-sensitivity targeted sequencing supports mixed clinical samples such as sputum and lavage fluid, enabling MTB detection without bacterial culture.
Streamlined Library Preparation
- The ATOPlex multiplex PCR platform enables target enrichment and library preparation in just two steps within a single tube, simplifying laboratory workflows.
Comprehensive Bioinformatics Analysis
- Based on the WHO catalogue of tuberculosis resistance mutations, MTB-Explorer software supports variant detection, resistance prediction, strain typing and evolutionary analysis across thousands of MTB mutations.
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