RNA-Seq Sequencing Combination Product for Large Populations
MGI’s RNA-Seq Sequencing Combination Product delivers a fully integrated solution for large-scale transcriptomics research. From nucleic acid extraction to library preparation, automated sample handling, high-throughput sequencing, and streamlined data analysis, this complete system covers every step of the RNA-Seq workflow. Built on MGI’s proprietary technologies, it combines speed, accuracy, and scalability, enabling researchers to process thousands of samples with consistency and minimal manual intervention. Whether for population cohorts, large-scale studies, or high-demand research environments, this product empowers scientists with reliable performance and the flexibility to meet diverse project needs.
Workflow
| Step 1: Sample Extraction | Step 2: Library Preparation | Step 3: Sequencing | Step 4: Data Analysis |
|---|---|---|---|
Automated Instruments:
Extraction Reagent:
| Library Prep Kits:
| Sequencer:
Sequencing Reagent:
| Hardware:
|
Performance Parameters
| Sample type | Fresh whole blood |
| Sample volume | ≥2 mL |
| Sample input | 200 μL |
| Nucleic acid type | Total RNA |
| Concentration | 10–50 ng/μL |
| Purity | 1.8 ≤ OD260/OD280 ≤ 2.0; OD260/OD230 ≥ 2 |
| Sample integrity | RIN ≥7 |
| Input amount | RIN ≥7: 200 ng; RIN <7: 500 ng |
Product Specifications
| Category | Product NO. | Product Name | Quantity |
|---|---|---|---|
| Extraction Reagents | 940-001760-00 | MGIEasy rRNA & Globin Depletion Kit V1.3 (96RXN) | / |
| Library Preps | 940-000888-00 | MGIEasy Fast RNA Library Prep Kit | / |
| 940-001763-00 | MGIEasy UDB Primers Adapter Kit A V2.1 (96RXN) | / | |
| 940-001762-00 | MGIEasy UDB Primers Adapter Kit B V2.1 (96RXN) | / | |
| 940-001748-00 | MGIEasy UDB Primers Adapter Kit C (96RXN) | / | |
| 1000020570 | MGIEasy Dual Barcode Circularization Kit | / | |
| 1000005279 | MGIEasy DNA Clean Beads (50 mL) | / | |
| Sequencing Reagents | 940-000268-00 | T7RS High-throughput Sequencing Set (FCL PE150) V3.0 | / |
| 1000020834 | CPAS Barcode Primer 3 Reagent Kit | / | |
| Automation Instruments | 900-000147-00 | SP-960RS High-throughput Automated Sample Preparation System – Custom Config 2-V2 | 1 |
| 900-000503-00 | SP-Smart 8RS Automated Sample Preparation System – Config 4 | 1 | |
| Sequencers | 900-000242-00 | Genetic Sequencer T7RS | 1 |
| 900-000134-00 | DNB Loader MGIDL-T7RS | 2 | |
| Data Processing | 900-000444-00 | Data Center Appliance MGI-ZTRON-PRO-T500 | 1 |
System Parameters
Fast, flexible library preparation
- Consolidates multiple experimental steps into one, reducing time and manual labour
- Supports conventional and directional RNA library construction.
- High uniform coverage and strand-specific information for precise gene expression detection.
High-quality sequencing output
- DNBSEQ technology ensures high accuracy, low duplication rates, and minimal index hopping.
- T7 platform handles large-scale sample sequencing efficiently.
Fully automated system
- Library preparation is efficiently performed using MGI’s proprietary automated platforms.
Complete solution for large populations
- Includes extraction kits, library prep kits, automated systems, sequencing platform, and bioinformatics software
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📞(+86) 4000-688-114
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