SARS-CoV-2 Sequencing Package (V3.1)
The SARS-CoV-2 Sequencing Package (V3.1) is a complete, high-throughput solution for whole-genome sequencing of SARS-CoV-2. It combines MGI’s proprietary reagents, automated sample preparation, advanced sequencing platforms, and integrated analysis software into one streamlined workflow. Supporting both short-read (DNBSEQ) and long-read (CycloneSEQ) technologies, it enables rapid variant detection, lineage identification, and phylogenetic analysis with minimal manual input.
Product Components & Part Numbers
| Part Number | |
|---|---|
| DNBSEQ Solution | |
| Genetic Sequencer DNBSEQ-G99ARS | 900-000609-00 |
| DNA Sequencing Library Preparation System MGISP-100RS | 900-000206-00 |
| ATOPlex RNA Multiplex PCR-based Library Preparation Set V3.1 (96 RXN) | 940-000133-00 |
| G99RS High-throughput Sequencing Set (G99 FCL SE100/PE50) | 940-001268-00 |
| DNBSEQ OneStep DNB Make Reagent Kit (OS-DB) (4 RXN) | 1000026466 |
| MGI metargetCOVID software | 970-000228-00 |
| CycloneSEQ Solution | |
| Nanopore Gene Sequencer Bundle 2 (CycloneSEQ-WT02 sequencer + workstation + keyboard & mouse + display screen) | H900-000003-00 |
| DNA Sequencing Library Preparation System SP-100RS | 900-000206-00 |
| MGIEasy Nucleic Acid Extraction Kit (96 RXN) | 1000020471 |
| ATOPlex RNA Multiplex PCR Amplification Set V3.1 (96 RXN) | 940-000135-00 |
| MGIEasy DNA Fast Library Prep Set (for CycloneSEQ) (24 RXN) | 940-002654-00 |
| WT Sequencing Flow Cell (2 pcs/set) | H930-000002-00 |
| CycloneSEQ WT Sequencing Kit (6 T) | H940-000016 |
| ATOPlex MultiPCR Microbiome Research Software (ATOPlex MMRS) | 970-000518-00 |
Performance & Workflow Parameters
| Parameter | DNBSEQ Solution | CycloneSEQ Solution |
| Sample Type | Throat swab, bronchoalveolar lavage fluid, etc. | Throat swab, bronchoalveolar lavage fluid, etc. |
| Amplification Area | SARS-CoV-2 full genome | SARS-CoV-2 full genome |
| Amplicon Length | 162 bp ~ 183 bp (average 178 bp) | 162 bp ~ 183 bp (average 178 bp) |
| Amplicon Number | 334 pairs (328 SARS-CoV-2, 4 external refs, 2 internal refs) | 334 pairs (328 SARS-CoV-2, 4 external refs, 2 internal refs) |
| Number of Amplification Tubes | 2 tubes | 2 tubes |
| Recommended RNA Input | 10 µL ~ 20 µL, Ct ≤ 35 | 10 µL ~ 20 µL, Ct ≤ 32 (stricter input requirement) |
| Automatic Library Preparation | SP-100 | SP-100 / CyclonePrep-4 |
| Library Preparation Time | 6 hours | 3.5 hours |
| Sequencing Platform | E25, G99, G50 | CycloneSEQ-WT02 |
| Sequencing Read Length | SE100+10+10 | Not applicable (long-read sequencing) |
| Bioinformatics Analysis Software | MGI metargetCOVID / ATOPlex MMRS | ATOPlex MMRS |
| Analysis Data Volume per Sample | 1–5M reads | 500M bases (unit differs: reads vs bases) |
| Detection Type | SNV, INDEL | SNV, INDEL |
| Solution Advantages | Cost-effective | Real-time sequencing for faster turnaround |
| Server Compatibility | PFI / PFI Pro / G99ARS | PFI / PFI Pro / CycloneSEQ-WT02 Workstation |
Key Features
Complete Workflow
Seamlessly manage everything from sample prep to data analysis in one streamlined package.
High Throughput
Effortlessly process hundreds of samples daily without sacrificing accuracy.
Dual Platform Support
Compatible with both short-read DNBSEQ and long-read CycloneSEQ sequencing technologies.
Accurate Variant Detection
Precisely identify mutations and variants with high sensitivity and confidence.
Automated Processing
Reduce manual steps and human error with automated sample handling and data workflows.
Fast Turnaround
Get rapid sequencing results to support timely public health decisions.
Discover More
Get in Touch
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📞(+86) 4000-688-114
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