G99: Lightning-Fast, High-Precision
The G99 is one of the fastest mid-to-low throughput sequencers on the market. Engineered with innovations in biochemistry, optics, fluidics, and temperature control, it delivers rapid, high-quality results without compromise.
Designed for flexibility, the G99 supports a wide range of sequencing applications and sample volumes. With multiple flow cell options, you can tailor your run to your project needs. Achieve paired-end 150 (PE150) sequencing with Q40 accuracy in just 12 hours, dramatically reducing turnaround times and increasing lab efficiency.
Other Material
Product Overview
Performance Parameters
| Max no of Flow Cells | Lane/ Flow Cell | Flow Cell Type | Reads/Flow Cell* | Supported Read Lengths | Data Output | Q30*** | Q40*** | Run Time*** |
|---|---|---|---|---|---|---|---|---|
| 2 | 1 | FCN | 20 M | App-D SE100 | 2 Gb~4 Gb | >90% | >85% | ~2.7 h |
| App-D PE150 | 6 Gb~12 Gb | >90% | >85% | ~6.7 h | ||||
| App-D PE300 | 12 Gb~24 Gb | >85% | >80% | ~18 h | ||||
| FCS | 40 M | App-D SE100 | 4 Gb~8 Gb | >90% | >85% | ~4 h | ||
| App-D PE150 | 12 Gb~24 Gb | >90% | >85% | ~11 h | ||||
| FCL | 80 M | SE100/PE50 | 8 Gb~16 Gb | >90% | >85% | ~5 h | ||
| PE150 | 24 Gb~48 Gb | >90% | >85% | ~12 h | ||||
| App-D SE100 | 8 Gb~16 Gb | >90% | >85% | ~5 h | ||||
| App-D PE150 | 24 Gb~48 Gb | >90% | >85% | ~12 h | ||||
| App-D PE300 | 48 Gb~96 Gb | >85% | >85% | ~30 h | ||||
| SE400 | 32 Gb~64 Gb | >75% | >70% | ~20 h | ||||
| FCU | 200 M | App-D SE100 | 20 Gb~40 Gb | >90% | >85% | ~7 h | ||
| App-D PE150 | 60 Gb~120 Gb | >85% | >85% | ~16 h | ||||
| App-D PE300 | 120 Gb~240 Gb | >85% | >80% | ~35 h | ||||
* Effective reads is determined using a standard library. Actual output may vary depending on sample type and library preparation method. ** The percentage of bases above Q30 and run time is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length. Only StandardMPS 2.0 (SM 2.0) reagents support the generation of Q40 data. *** The sequencing time is the statistical duration for single flow cell sequencing and dual flow cell simultaneous sequencing. ★ The instrument is equipped with SE50 and PE100 sequencing modes, and the existing reagent kits support SE50 and PE100 read length sequencing. Note: APP-D has built-in Illumina’s Truseq, Nextera adapters, and MGI adapter, which supporting mixed testing of Illumina’s Truseq, Nextera adapters, and MGI adapter libraries. | ||||||||
| Method & Application Recommendations | ||
|---|---|---|
| Method | Application | Recommended Read Length / Sample / Run |
| Target capture / multiplex PCR | Onco panel | PE100/PE150 FCN: 6/FC, 12/RUN |
| Genetic disease diagnosis small panel | Deafness | PE150 FCN: 1/FC, 2/RUN |
| Thalassemia | PE150 FCN: 100/FC, 200/RUN | |
| ATOPlex panel | Respiratory, COVID-19, etc. | PE100/PE150 FCN: 4/FC，8/RUN |
| WES | Whole Exome Sequencing | PE150 15 Gb/sample FCL: 1–2/FC, 2–4/RUN FCU: 4/FC, 8/RUN |
| Methylation | Onco targeted methylation panel | PE150 FCN: 1/FC，2/RUN |
| Small genome sequencing | Metagenomics for pathogen detection | SE50/SE100 FCN: 1/FC，2/RUN |
| Microbial WGS sequencing | PE100/PE150 FCN: 6/FC，12/RUN | |
| 16S sequencing | PE300 FCN: 160/FC，320/RUN | |
| Low-pass WGS sequencing | NIPT | SE50 ~10 M reads/sample FCL: 8/FC, 16/RUN FCU: 20/FC, 40/RUN |
| PGS | SE50 – FCL: – FCU: – | |
| Transcriptome sequencing | RNA-Seq Expression profiling | SE50/PE150 ~25 M reads/sample FCL: 3/FC, 6/RUN FCU: 8/FC, 16/RUN |
| Transcriptome | SE50/PE150 ~6 Gb/sample FCL: 4/FC, 8/RUN FCU: 10/FC, 20/RUN | |
| Forensics | DNA signature identification | SE400 ~0.8 M reads/sample FCL: 96/FC, 192/RUN |
System Parameters
Product Model
- G99RS: Stable output of standard FASTQ files from 100 V to 240 V.
- G99ARS: Equipped with a bioinformatics computing module, it can perform advanced analysis.
Power, Dimensions & Net Weight
- Power Type: 100 V–240 V, 50/60 Hz
- Rated Power: 1,000 VA (with a current supply of not less than 10 A)
- Dimensions (W×D×H): 689 mm × 607 mm × 657 mm
- Net Weight: ~140 kg
- Maximum sound pressure level*: 75 dB(A)
- Enclosure protection rating: IPX0
Operating Environment Requirements
- Temperature: 15℃–30℃
- Relative Humidity: 20% RH–80% RH, non-condensing
- Atmospheric Pressure: 70 kPa–106 kPa
- Maximum altitude: 3000m
*For indoor use only.
Bioinformatics Module Configurations
- CPU: Intel Xeon 5220S 18C/36T 2.7GHz*
- Memory: 256 GB
- System Disk: 960 GB
- Cache Disk: 960 GB
- Hard Drive: 32 TB
- Cloud Storage: Gigabit RJ45 interfaces *2
Computer Configurations
- CPU: Intel i9-10900e 2.8 GHz
- Memory: 64 GB
- SSD: 5.76 TB
- Operating System: Windows 10
* The maximum sound pressure level is measured and calculated at any position 1 metre away from the enclosure during normal operation.
** For indoor use only.
*** Supports computer configuration and system version upgrades, etc.
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