Microbiome Metabarcoding Sequencing Package

Microorganisms exist in nearly every environment, from humans to soil, plants, and industrial settings, playing key roles in ecosystems. MGI’s Microbiome Metabarcoding Sequencing Package provides a complete workflow, combining nucleic acid extraction reagents, ATOPlex library prep, SP-960 automation, G99 and E25 sequencing and MetaSIS analysis software.

It enables rapid, accurate profiling of microbial communities in environmental, human, industrial, and agricultural samples, supporting research and practical applications in health, ecology and industry.

Product Components & Part Numbers

Item No.	Product Name
900-000609-00	G99ARS (Include server)
900-000537-00	E25RS (Standard Config)
950-000020-00	SP-NE32RS
900-000154-00	SP-960RS High-throughput Automated Sample Preparation System (Configuration 9)
940-001247-00	MGIEasy Stool DNA (Meta) Extraction Kit V2.0 (96 Preps)
940-001246-00	MGIEasy Stool DNA (Meta) Extraction Kit V2.0 (384 Preps)
940-000136-00	MGIEasy Tissue Grinding Beads (96 Preps/Bottle) View the Datasheet
	ATOPlex Library Preparation Set
1000020570	MGIEasy Dual Barcode Circularization Kit V1.0 (16 RXN)
1000026466	DNBSEQ OneStep DNB Make Reagent Kit (4 RXN)
940-001269-00	High-throughput Sequencing Set (G99 FCL PE150)
940-001716-00	High-throughput Sequencing Set (G99 App-D FCL PE300)
940-000567-00	E25RS High-throughput Sequencing Set (FCL PE150)
970-000417-00	Metabarcoding Species Identification Software
970-000456-00	Metabarcoding Species Identification Software Package (96 reports)
900-000393-00	Platform of microorganisms Fast Identification
900-000399-00	Platform of microorganisms Fast Identification and assembly evolution

Product Specification

Recommended Sample Types	Feces, oral swabs, skin swabs, tongue coating swabs, saliva, rhizosphere soil, leaf surfaces, fermented grains, yogurt, sediment, etc.
Applicable Species	Bacteria, Fungi
Recommended Sample Input	DNA: 10 ng
Recommended Sequencing Read Length	16SV3V4/ITS1/ITS2: PE300; 16SV4: PE150
Recommended Data Volume	≥65K reads per sample
Recommended Maximum Sequencing Throughput	G99 PE150: 500 Samples / FC G99 PE300: 300 Samples / FC E25 PE150: 72 Samples / FC
Shortest Reporting Period	20.5 hrs

System Parameters

Versatile Application Scenarios

Designed around popular rDNA variable regions, compatible with diverse sample types for human, industrial, agricultural, and environmental microbiome research.

One-Stop Solution

Integrated workflow from nucleic acid extraction to library prep, sequencing, and analysis, providing all the tools needed in a single package..

High-Quality Data

Unique DNBSEQ sequencing technology ensures accurate, reliable, and high-quality sequencing results for confident microbial profiling.

Powerful Analysis & Scalability

Self-developed software supports OTU, species composition, Alpha/Beta diversity, biomarker analysis, and customized databases. Up to 4,608 barcodes enable ultra-high throughput sequencing at reduced cost.