Respiratory Microbial Genomic Sequencing Package
MGI’s Respiratory Microbial Genomic Sequencing Package delivers fast, accurate, and comprehensive sequencing of respiratory pathogens, with a dedicated focus on Influenza A/B. This end-to-end solution combines multiPCR enrichment, automated sample preparation, high-throughput sequencing, and integrated bioinformatics, enabling near-fully automated analysis with minimal hands-on time.
The package amplifies all 8 genomic segments of Influenza A (HA subtypes H1–H16, NA subtypes N1–N9) and Influenza B (Victoria and Yamagata lineages), providing complete viral genome coverage. The resulting data supports typing, genome assembly, and traceability analysis, offering critical insights for influenza research and surveillance.
Product Lineup & Part Numbers
| Product | Part Number / Details |
|---|---|
| DNBSEQ Targeted Sequencing Solution | |
| Genetic Sequencer G99 / E25 / SEQ-200 | – |
| DNA Sequencing Library Preparation System SP-100 / SP-960 / D4 | – |
| Automated Nucleic Acid Extraction System SP-NE32 | – |
| MGIEasy DNA Extraction Reagent | – |
| MGIEasy Respiratory Microbial Genomic Library Preparation Kit | – |
| Sequencing Read Length | PE100 + 10bp dual indexes |
| Bioinformatics Software – MGI FluTrack (vX.X) | – |
| Microbial ID Platform – PFI / G99ARS | – |
| CycloneSEQ Targeted Sequencing Solution | |
| Nanopore Gene Sequencer Bundle 2 (CycloneSEQ-WT02 + accessories) | H900-000003-00 |
| DNA Sequencing Library Preparation System MGISP-100RS | 900-000206-00 |
| High-throughput Automated Sample Preparation System MGISP-960RS Config7 | 900-000152-00 |
| MGIEasy Microbiome DNA Extraction Kit MD01T-96 (96 RXN) | 1000027955 |
| MGIEasy DNA Fast Library Prep Set (for CycloneSEQ) (24 RXN) | 940-002654-00 |
| WT Sequencing Flow Cell (2 pcs/set) | H930-000002-00 |
| CycloneSEQ WT Sequencing Kit (6 T) | H940-000016 |
| ATOPlex MultiPCR Microbiome Research Software (ATOPlex MMRS) | 970-000518-00 |
Technical Specifications
| Specification | Details |
|---|---|
| Target Regions | Full-length amplification of all 8 segments (H1–H16, N1–N9 subtypes, B Victoria and Yamagata lineages) |
| Amplicon Length | 0.8–2.4 kb |
| Primer Design | Degenerate influenza primers with internal/external controls |
| Number of Amplification Tubes | 1 tube |
| RNA Input | ≤18.5 μL (10 μL recommended) |
| Sample Types | Nasopharyngeal swabs, virus cultures, etc. |
| Sequencing Mode | PE100+10+10 |
| Data Analysis | 5 M reads per sample |
Workflow
| Workflow Step | Details |
|---|---|
| Nucleic Acid Extraction | • MGIEasy Nucleic Acid Extraction Kit • SP-NE32 |
| Library Preparation | • MGIEasy Respiratory Microorganisms Genome Library Preparation Set • SP-100/SP-960/D4 |
| Sequencing | • G99/E25 • G50 • Sequencing Read Length: PE100 |
| Bioinformatics Analysis | • MGI FluTrack • PFI/G99ARS |
Key Features
Seamless Integration
Complete sample-to-report workflow with automated prep, sequencing, and analysis.
Exceptional Accuracy
99% on-target rate and uniform coverage for reliable influenza genomic data.
Effortless Automation
Automated sample prep and data processing minimize hands-on time and errors.
Advanced Sequencing Tech
Powered by MGI’s exclusive DNBSEQ™ and cPAS technologies for superior results.
Comprehensive Genome Coverage
Full-length amplification of all 8 influenza genome segments, subtypes included.
Rapid Turnaround
Fast, high-throughput sequencing accelerates your influenza surveillance and research.
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Customer Stories
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