Stereo-seq Visualization Reagent Set

MGI’s Stereo-seq Visualization Reagent Set leverages cPAS and DNBSEQ™ technologies for STOmics libraries, delivering high-performance sequencing for Stereo-seq library DNBSEQ-T7RS and DNBSEQ-G400RS platforms. This all-in-one kit includes library sequencing primers, one-step DNB preparation reagents, and pre-packaged components, optimized with StandardMPS 2.0 chemistry for improved data quality. It supports the complete workflow of the Stereo-seq FF library (v1.3), the Stereo-CITE library (V1.1), and the Stereo-seq FFPE library (V1.0 and V1.1), providing a seamless end-to-end solution.

Product Overview

Product Specifications

DNBSEQ Stereo-seq Visualization Reagent Sets
	DNBSEQ-G400RS Stereo-seq Visualization Reagent Set	DNBSEQ-T7RS Stereo-seq Visualization Reagent Set
Read length	PE75	PE75
Flow Cell Type	FCL	FCL
Lane No./Flow Cell	4	1
Effective reads/Flow Cell*	1280 M ~ 1800 M	4500 M ~ 5800 M
Q30**	>85%	>85%
Run time	22 h***	12 – 20 h
Item No.	940-001886-00 (G400 STO FCL PE75)	940-001895-00 (T7 STO FCL PE75)
* The effective reads value is obtained according to the operation of a specific standard library, and the actual application library will fluctuate according to the sample type and library construction method.
** The percentage of bases above Q30 and the running time are averaged over the entire run for a particular standard library. The practical application performance is affected by sample type, library quality, insert length and other factors.
*** Run time is calculated based on dual-slide mode including sample loading, sequencing, base calling, and data processing (From Post loading prime to output FASTQ file).
**** Run time includes Flow Cell loading, sequencing, and outputting Cal. File. Cal. is a binary file format generated by MGI sequencer basecall software.

Workflow

System Parameters

Dual-Platform Adapted

Optimized for the Stereo-seq library workflow, delivering high-performance sequencing on both DNBSEQ-T7RS and DNBSEQ-G400RS platforms..

Highly Integrated

Includes primers and OneStep DNB reagents pre-packed in the kit for streamlined, easy-to-use workflows.

STOmics Libraries Exclusive

Designed specifically for Stereo-seq libraries, the Stereo-seq visualization reagent set is fully compatible with Stereo-seq FF (v1.3), Stereo-CITE (v1.1), and Stereo-seq FFPE (v1.0 and v1.1).
DNBSEQ-T7RS stereo-seq visualisation reagent set also supports mixed sequencing of Stereo-seq libraries, including Stereo-seq FFPE v1.1 with Stereo-seq FF v1.3, as well as Stereo-CITE v1.1 cDNA with Stereo-CITE v1.1 ADT libraries.