Whole-Genome Methylation Sequencing (WGMS) Combination Product for Large Populations

The Whole Genome Methylation Sequencing (WGMS) Combination Product is designed for large-scale population studies, offering an end-to-end solution from blood sampling to methylation profiling and VCF output. Leveraging MGI’s proprietary WGMS library preparation reagents, automated sample preparation systems, DNBSEQ high-throughput sequencing platform, and comprehensive bioinformatics analysis software, it fully automates the experimental and data processing workflow.

This integrated solution ensures high-depth, full-genome methylation sequencing with exceptional data quality. Features include no library balancing requirements, high genome coverage, accurate methylation detection, and efficient sequencing with low duplication and minimal index hopping.

Workflow

Step 1: Sample Extraction	Step 2: Library Preparation	Step 3: Sequencing	Step 4: Data Analysis
Automated Instruments: SP-960RS Extraction Reagent: MGIEasy Magnetic Beads Blood Genomic DNA Extraction Kit (V1.0)	Automated Instruments: SP-Smart 8RS SP-960RS Library Prep Kits: MGIEasy Whole Genome Methylation Sequencing Library Prep Kit NEBNext Enzymatic Methyl-seq Conversion Module Formamide, Diamond Unmethylated Lambda DNA, Promega	Sequencer: T7RS Sequencing Reagent: T7RS Methylation High-throughput Sequencing Set (T7 Methyl FCL PE150)	Hardware: Data Center Appliance ZTRON-PRO-T500 Software: ZBOLT Pro Bioinformatics Analysis Accelerator MethyScope Software

Step 1: Sample Extraction

Step 2: Library Preparation

Step 3: Sequencing

Step 4: Data Analysis

Automated Instruments:

SP-960RS

Extraction Reagent:

MGIEasy Magnetic Beads Blood Genomic DNA Extraction Kit (V1.0)

Automated Instruments:

SP-Smart 8RS
SP-960RS

Library Prep Kits:

MGIEasy Whole Genome Methylation Sequencing Library Prep Kit
NEBNext Enzymatic Methyl-seq Conversion Module
Formamide, Diamond
Unmethylated Lambda DNA, Promega

Sequencer:

T7RS

Sequencing Reagent:

T7RS Methylation High-throughput Sequencing Set (T7 Methyl FCL PE150)

Hardware:

Data Center Appliance ZTRON-PRO-T500

Software:

ZBOLT Pro Bioinformatics Analysis Accelerator
MethyScope Software

Performance Parameters

Sample Type	Whole blood
Sample Volume	≥2 mL
Sample Input	140 μL
Nucleic Acid Type	gDNA
Concentration	≥11.5 ng/μL
Purity	1.8 ≤ OD260/OD280 ≤ 2.0; OD260/OD230 ≥ 1.7
Sample Integrity	No degradation or slight degradation (e.g., electrophoresis)
Input Amount	200 ng gDNA

Product Specifications

Category	Product No.	Product Name	Qty
Extraction Reagents	940-000633-00	MGIEasy Magnetic Beads Blood Genomic DNA Extraction Kit (V1.0)	/
Library Preps	940-001527-00	MGIEasy Whole Genome Methylation Sequencing Library Prep Kit	/
	E7125L	NEBNext Enzymatic Methyl-seq Conversion Module	/
	A100314-0100	Formamide, Diamond	/
	D1521	Unmethylated Lambda DNA, Promega	/
Sequencing Reagents	940-002625-00	T7RS Methylation High-throughput Sequencing Set (T7 Methyl FCL PE150)	/
Automation Instruments	900-000152-00	SP-960RS High-throughput Automated Sample Preparation System-Configuration 7	1
Automation Instruments	900-000500-00	Automated Sample Preparation System SP-Smart 8RS Config 2	1
Sequencers	900-000242-00	Genetic Sequencer T7RS	1
Sequencers	900-000134-00	DNB Loader MGIDL-T7RS	2
Data Processing	900-000444-00	Data Center Appliance MGI-ZTRON-PRO-T500	1
	900-000460-00	ZBOLT Pro Bioinformatics Analysis Accelerator	1
	970-000441-00	MethyScope Software	1

System Parameters

No Library Balancing Required

MGIEasy Whole-Genome Methylation Library Preparation Kit plus the DNBSEQ upgraded algorithm eliminates the need for library balancing.
Improves data yield, genome coverage, and methylation accuracy.

Efficient and High-Quality Sequencing Output

DNBSEQ technology delivers high accuracy, low duplication rates, and minimal index hopping.
Scalable for large sample sets.

Fully Automated Workflow

Automated systems handle nucleic acid extraction and library prep efficiently.
Reduces hands-on time and increases reproducibility.

Comprehensive WGMS Solution

Includes extraction and library prep kits, automated instruments, sequencing platforms, and bioinformatics software.
Supports population-scale studies with flexibility for reagents, consumables, and hardware options.