Shenzhen, China, 5 September 2025 — MGI Tech Co., Ltd. (“MGI”), a company dedicated to developing core tools and technologies that drive innovation in life sciences, announced the extension of applications of MGIEasy Fast FS Library Prep Set V2.0. This breakthrough enables ultra-low DNA input library preparation, enhancing experimental flexibility and providing a wider range of application scenarios for research exploration.
MGIEasy Fast FS Library Prep Set V2.0 combines fragmentation, end-repair, and A-tailing into a single step, simplifying the preparation process and significantly reducing DNA library preparation time to just 2 hours. Recently, species abundance in trace metagenomic DNA samples ranging from 1 pg to 10 pg has been effectively detected using the optimized ultra-low DNA input library preparation workflow.
This innovative system enables efficient and rapid library preparation for trace samples, demonstrating comprehensive performance in ultra-low input applications while greatly improving the utilization of precious samples. Additionally, it provides a robust library preparation solution for analysing microbial community abundance and composition in trace samples from challenging scenarios, including extreme environments and ecological research.
Key Features
MGIEasy Fast FS Library Prep Set V2.0 features several highlights that ensure a streamlined library preparation workflow:
- Breaking Trace Sample Input Limitations: Enables library preparation from trace samples as low as 1 pg
- High-Quality Library Preparation: Optimized workflow ensures high-quality library construction with extremely low sample input amounts
- High Sensitivity and Accuracy: Delivers excellent sequencing quality and accurate detection of low-abundance nucleic acid sequences
Performance Demonstration
1. High-Quality Library Preparation
The MGIEasy Fast FS Library Prep Set ultra-low input workflow was evaluated using varied initial DNA inputs (1 pg, 5 pg, and 10 pg) based on the ZymoBIOMICS™ Microbial Community DNA Standard.
Library Yield Results:
• 1 pg input: ~95 ng yield (22 PCR cycles)
• 5 pg input: ~100 ng yield (20 PCR cycles)
• 10 pg input: ~190 ng yield (20 PCR cycles)
These results demonstrate efficient utilization of trace samples and suitability for downstream high-throughput sequencing. Agilent 2100 Bioanalyzer analysis confirmed consistent fragment distributions with no primer dimers or non-specific peaks.
2. Sequencing Quality Performance
Library sequencing on the DNBSEQ-G400 platform (PE150) produced high-quality data:
Sequencing Metrics:
• Total reads: 515 million
• Q30 score: > 90 %
• Split rate: > 95 %
Base quality remained stable across all sequencing cycles, supporting deep downstream analyses.
3. Stable Performance Indicators
Species abundance analysis was performed on one replicate per input group (1 pg, 5 pg, 10 pg). The results were consistent with theoretical expectations:
Species Detection at 1 pg Input:
Pseudomonas aeruginosa (14.61 %), Escherichia coli (11.64 %), Salmonella enterica (11.81 %), Lactobacillus fermentum (11.02 %), Enterococcus faecalis (11.33 %), Staphylococcus aureus (11.38 %), Listeria monocytogenes (10.48 %), Bacillus subtilis (10.65 %), Saccharomyces cerevisiae (1.47 %), Cryptococcus neoformans (1.38 %).
Correlation Analysis Results:
• 1 pg: 0.9927
• 5 pg: 0.9953
• 10 pg: 0.9942
All coefficients exceeded 0.99, indicating highly consistent species abundance distributions across input ranges.
Applications
The MGIEasy Fast FS Library Prep Set V2.0 provides comprehensive solutions for diverse research applications:
- Extreme Environment Research: Microbial community analysis in challenging conditions
- Ecological Studies: Abundance and composition analysis in trace environmental samples
- Precious Sample Utilization: Maximizing data output from limited specimens
- Clinical Diagnostics: Detection of low-abundance pathogens
- Forensic Applications: DNA analysis from degraded or trace evidence
Available Product Configurations:
- MGIEasy Fast FS Library Prep Set V2.0 with UDB Primers Adapter Kit A
- MGIEasy Fast FS Library Prep Set V2.0 with UDB Primers Adapter Kit B
- MGIEasy Fast FS Library Prep Set V2.0 with UDB Primers Adapter Kit C
For more information on our complete range of library preparation kits, please visit: MGI Tech Library Preparation Kits
